In vivo evaluation of ovine sperm/embryo ability in mediating transgenic lamb
Here, we evaluated the ovine embryo ability in DNA uptake as an alternate pathway for sperm-mediated transgenesis. EGFP carrying plasmid was intra-uterus injected through laparoscopy either five days following natural insemination or mixed with sperm cells at the time of fertilization. Excluding tri...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2015-12-01
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| Series: | Middle East Fertility Society Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110569014200343 |