Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant Activity
Biogenic selenium nanoparticles (SeNPs) have been shown to exhibit increased bioavailability. Fermentation of pollen by a symbiotic culture of bacteria and yeasts (SCOBY/Kombucha) leads to the release of pollen content and enhances the prebiotic and probiotic effects of Kombucha. The aim of this stu...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/9/1711 |
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| author | Naomi Tritean Ștefan-Ovidiu Dima Bogdan Trică Rusăndica Stoica Marius Ghiurea Ionuț Moraru Anisoara Cimpean Florin Oancea Diana Constantinescu-Aruxandei |
| author_facet | Naomi Tritean Ștefan-Ovidiu Dima Bogdan Trică Rusăndica Stoica Marius Ghiurea Ionuț Moraru Anisoara Cimpean Florin Oancea Diana Constantinescu-Aruxandei |
| author_sort | Naomi Tritean |
| collection | DOAJ |
| description | Biogenic selenium nanoparticles (SeNPs) have been shown to exhibit increased bioavailability. Fermentation of pollen by a symbiotic culture of bacteria and yeasts (SCOBY/Kombucha) leads to the release of pollen content and enhances the prebiotic and probiotic effects of Kombucha. The aim of this study was to fortify Kombucha beverage with SeNPs formed in situ by Kombucha fermentation with pollen. Response Surface Methodology (RSM) was used to optimize the biosynthesis of SeNPs and the pollen-fermented Kombucha beverage. SeNPs were characterized by Transmission electron microscopy energy-dispersive X-ray spectroscopy (TEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), and Zeta potential. The pollen-fermented Kombucha beverage enriched with SeNPs was characterized by measuring the total phenolic content, antioxidant activity, soluble silicon, saccharides, lactic acid, and the total content of Se<sup>0</sup>. The polyphenols were identified by liquid chromatography–mass spectrometry (LC-MS). The pollen and the bacterial (nano)cellulose were characterized by scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), FTIR, and X-Ray diffraction (XRD). We also assessed the in vitro biocompatibility in terms of gingival fibroblast viability and proliferation, as well as the antioxidant activity of SeNPs and the pollen-fermented Kombucha beverage enriched with SeNPs. The results highlight their increased biological performance in this regard. |
| first_indexed | 2024-03-10T23:06:07Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T23:06:07Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-146c0c92becd4e72a87777d61e9f94582023-11-19T09:19:21ZengMDPI AGAntioxidants2076-39212023-09-01129171110.3390/antiox12091711Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant ActivityNaomi Tritean0Ștefan-Ovidiu Dima1Bogdan Trică2Rusăndica Stoica3Marius Ghiurea4Ionuț Moraru5Anisoara Cimpean6Florin Oancea7Diana Constantinescu-Aruxandei8Bioresources, Polymers and Analysis Departments, National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, Splaiul Independenței No. 202, Sector 6, 060021 Bucharest, RomaniaBioresources, Polymers and Analysis Departments, National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, Splaiul Independenței No. 202, Sector 6, 060021 Bucharest, RomaniaBioresources, Polymers and Analysis Departments, National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, Splaiul Independenței No. 202, Sector 6, 060021 Bucharest, RomaniaBioresources, Polymers and Analysis Departments, National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, Splaiul Independenței No. 202, Sector 6, 060021 Bucharest, RomaniaBioresources, Polymers and Analysis Departments, National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, Splaiul Independenței No. 202, Sector 6, 060021 Bucharest, RomaniaMedica Laboratories, Str. Frasinului nr. 11, 075100 Otopeni, RomaniaFaculty of Biology, University of Bucharest, Splaiul Independentei No. 91-95, 050095 Bucharest, RomaniaBioresources, Polymers and Analysis Departments, National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, Splaiul Independenței No. 202, Sector 6, 060021 Bucharest, RomaniaBioresources, Polymers and Analysis Departments, National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, Splaiul Independenței No. 202, Sector 6, 060021 Bucharest, RomaniaBiogenic selenium nanoparticles (SeNPs) have been shown to exhibit increased bioavailability. Fermentation of pollen by a symbiotic culture of bacteria and yeasts (SCOBY/Kombucha) leads to the release of pollen content and enhances the prebiotic and probiotic effects of Kombucha. The aim of this study was to fortify Kombucha beverage with SeNPs formed in situ by Kombucha fermentation with pollen. Response Surface Methodology (RSM) was used to optimize the biosynthesis of SeNPs and the pollen-fermented Kombucha beverage. SeNPs were characterized by Transmission electron microscopy energy-dispersive X-ray spectroscopy (TEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), and Zeta potential. The pollen-fermented Kombucha beverage enriched with SeNPs was characterized by measuring the total phenolic content, antioxidant activity, soluble silicon, saccharides, lactic acid, and the total content of Se<sup>0</sup>. The polyphenols were identified by liquid chromatography–mass spectrometry (LC-MS). The pollen and the bacterial (nano)cellulose were characterized by scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), FTIR, and X-Ray diffraction (XRD). We also assessed the in vitro biocompatibility in terms of gingival fibroblast viability and proliferation, as well as the antioxidant activity of SeNPs and the pollen-fermented Kombucha beverage enriched with SeNPs. The results highlight their increased biological performance in this regard.https://www.mdpi.com/2076-3921/12/9/1711biogenic nanoseleniumkombucha fermentationselenium-nanoparticles-enriched bee breadantioxidantbiocompatibleresponse surface methodology |
| spellingShingle | Naomi Tritean Ștefan-Ovidiu Dima Bogdan Trică Rusăndica Stoica Marius Ghiurea Ionuț Moraru Anisoara Cimpean Florin Oancea Diana Constantinescu-Aruxandei Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant Activity Antioxidants biogenic nanoselenium kombucha fermentation selenium-nanoparticles-enriched bee bread antioxidant biocompatible response surface methodology |
| title | Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant Activity |
| title_full | Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant Activity |
| title_fullStr | Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant Activity |
| title_full_unstemmed | Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant Activity |
| title_short | Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant Activity |
| title_sort | selenium fortified kombucha pollen beverage by in situ biosynthesized selenium nanoparticles with high biocompatibility and antioxidant activity |
| topic | biogenic nanoselenium kombucha fermentation selenium-nanoparticles-enriched bee bread antioxidant biocompatible response surface methodology |
| url | https://www.mdpi.com/2076-3921/12/9/1711 |
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