Differences in melting temperatures of degenerated oligonucleotides targetting nitrous oxide reductase (nosZ) genes
One of the basic principles of molecular biology is the use oligonucleotides with comparable melting temperatures (Tm). To accommodate various evolutionary changes in target gene sequences in order to detect numerous variants of the same gene in complex microbial communities, the researchers were f...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Ljubljana Press (Založba Univerze v Ljubljani)
2008-11-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/15091 |