Weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest ‘Bingtangcheng’ and ‘Tarocco’ blood orange fruit
Abstract Background Organic acids and anthocyanins are the most important compounds for the flavor and nutritional quality of citrus fruit. However, there are few reports on the involvement of co-regulation of citrate and anthocyanin metabolism. Here, we performed a comparative transcriptome analysi...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04309-5 |
| _version_ | 1797811523859513344 |
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| author | Yan Jin Manyu Liao Na Li Xiaoqian Ma Huimin Zhang Jian Han Dazhi Li Junfeng Yang Xiaopeng Lu Guiyou Long Ziniu Deng Ling Sheng |
| author_facet | Yan Jin Manyu Liao Na Li Xiaoqian Ma Huimin Zhang Jian Han Dazhi Li Junfeng Yang Xiaopeng Lu Guiyou Long Ziniu Deng Ling Sheng |
| author_sort | Yan Jin |
| collection | DOAJ |
| description | Abstract Background Organic acids and anthocyanins are the most important compounds for the flavor and nutritional quality of citrus fruit. However, there are few reports on the involvement of co-regulation of citrate and anthocyanin metabolism. Here, we performed a comparative transcriptome analysis to elucidate the genes and pathways involved in both citrate and anthocyanin accumulation in postharvest citrus fruit with ‘Tarocco’ blood orange (TBO; high accumulation) and ‘Bingtangcheng’ sweet orange (BTSO; low accumulation). Results A robust core set of 825 DEGs were found to be temporally associated with citrate and anthocyanin accumulation throughout the storage period through transcriptome analysis. Further according to the results of weighted gene coexpression correlation network analysis (WGCNA), the turquoise and brown module was highly positively correlated with both of the content of citrate and anthocyanin, and p-type ATPase (PH8), phosphoenolpyruvate carboxylase kinase (PEPCK), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3’-hydroxylase (F3’H) and glutathione S transferase (GST) were considered key structural genes. Moreover, MYB family transcription factor (PH4), Zinc finger PHD-type transcription factor (CHR4, HAC12), Zinc finger SWIM-type transcription factor (FAR1) and Zinc finger C3H1-type transcription factor (ATC3H64) were considered hub genes related to these structural genes. Further qRT-PCR analysis verified that these transcription factors were highly expressed in TBO fruit and their expression profiles were significantly positively correlated with the structural genes of citrate and anthocyanin metabolism as well as the content of citrate and anthocyanin content. Conclusions The findings suggest that the CHR4, FAR1, ATC3H64 and HAC12 may be the new transcription regulators participate in controlling the level of citrate and anthocyanin in postharvest TBO fruit in addition to PH4. These results may providing new insight into the regulation mechanism of citrate and anthocyanin accumulation in citrus fruit. |
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| format | Article |
| id | doaj.art-148c6a146f924a4db5bea3832faa1864 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-13T07:25:00Z |
| publishDate | 2023-06-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-148c6a146f924a4db5bea3832faa18642023-06-04T11:24:48ZengBMCBMC Plant Biology1471-22292023-06-0123111410.1186/s12870-023-04309-5Weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest ‘Bingtangcheng’ and ‘Tarocco’ blood orange fruitYan Jin0Manyu Liao1Na Li2Xiaoqian Ma3Huimin Zhang4Jian Han5Dazhi Li6Junfeng Yang7Xiaopeng Lu8Guiyou Long9Ziniu Deng10Ling Sheng11National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityHunan Horticultural Research InstituteNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityNational Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural UniversityAbstract Background Organic acids and anthocyanins are the most important compounds for the flavor and nutritional quality of citrus fruit. However, there are few reports on the involvement of co-regulation of citrate and anthocyanin metabolism. Here, we performed a comparative transcriptome analysis to elucidate the genes and pathways involved in both citrate and anthocyanin accumulation in postharvest citrus fruit with ‘Tarocco’ blood orange (TBO; high accumulation) and ‘Bingtangcheng’ sweet orange (BTSO; low accumulation). Results A robust core set of 825 DEGs were found to be temporally associated with citrate and anthocyanin accumulation throughout the storage period through transcriptome analysis. Further according to the results of weighted gene coexpression correlation network analysis (WGCNA), the turquoise and brown module was highly positively correlated with both of the content of citrate and anthocyanin, and p-type ATPase (PH8), phosphoenolpyruvate carboxylase kinase (PEPCK), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3’-hydroxylase (F3’H) and glutathione S transferase (GST) were considered key structural genes. Moreover, MYB family transcription factor (PH4), Zinc finger PHD-type transcription factor (CHR4, HAC12), Zinc finger SWIM-type transcription factor (FAR1) and Zinc finger C3H1-type transcription factor (ATC3H64) were considered hub genes related to these structural genes. Further qRT-PCR analysis verified that these transcription factors were highly expressed in TBO fruit and their expression profiles were significantly positively correlated with the structural genes of citrate and anthocyanin metabolism as well as the content of citrate and anthocyanin content. Conclusions The findings suggest that the CHR4, FAR1, ATC3H64 and HAC12 may be the new transcription regulators participate in controlling the level of citrate and anthocyanin in postharvest TBO fruit in addition to PH4. These results may providing new insight into the regulation mechanism of citrate and anthocyanin accumulation in citrus fruit.https://doi.org/10.1186/s12870-023-04309-5TranscriptomeAnthocyaninCitrate‘Bingtangcheng’ sweet orange‘Tarocco’ blood orangePostharvest |
| spellingShingle | Yan Jin Manyu Liao Na Li Xiaoqian Ma Huimin Zhang Jian Han Dazhi Li Junfeng Yang Xiaopeng Lu Guiyou Long Ziniu Deng Ling Sheng Weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest ‘Bingtangcheng’ and ‘Tarocco’ blood orange fruit BMC Plant Biology Transcriptome Anthocyanin Citrate ‘Bingtangcheng’ sweet orange ‘Tarocco’ blood orange Postharvest |
| title | Weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest ‘Bingtangcheng’ and ‘Tarocco’ blood orange fruit |
| title_full | Weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest ‘Bingtangcheng’ and ‘Tarocco’ blood orange fruit |
| title_fullStr | Weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest ‘Bingtangcheng’ and ‘Tarocco’ blood orange fruit |
| title_full_unstemmed | Weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest ‘Bingtangcheng’ and ‘Tarocco’ blood orange fruit |
| title_short | Weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest ‘Bingtangcheng’ and ‘Tarocco’ blood orange fruit |
| title_sort | weighted gene coexpression correlation network analysis reveals the potential molecular regulatory mechanism of citrate and anthocyanin accumulation between postharvest bingtangcheng and tarocco blood orange fruit |
| topic | Transcriptome Anthocyanin Citrate ‘Bingtangcheng’ sweet orange ‘Tarocco’ blood orange Postharvest |
| url | https://doi.org/10.1186/s12870-023-04309-5 |
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