Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone.
Glucocorticoid induced osteoporosis (GIO) is associated with an increase in bone marrow adiposity which skews the differentiation of mesenchymal stem cell (MSC) progenitors away from osteoblastogenesis and towards adipogenesis. We have previously found that vanadate, a non-specific protein tyrosine...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-08-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fendo.2016.00108/full |
| _version_ | 1819208088308154368 |
|---|---|
| author | Frans Alexander Jacobs Hanel Sadie-Van Gijsen Mari van de Vyver William Frank Ferris |
| author_facet | Frans Alexander Jacobs Hanel Sadie-Van Gijsen Mari van de Vyver William Frank Ferris |
| author_sort | Frans Alexander Jacobs |
| collection | DOAJ |
| description | Glucocorticoid induced osteoporosis (GIO) is associated with an increase in bone marrow adiposity which skews the differentiation of mesenchymal stem cell (MSC) progenitors away from osteoblastogenesis and towards adipogenesis. We have previously found that vanadate, a non-specific protein tyrosine phosphatase inhibitor, prevents GIO in rats, but it was unclear whether vanadate directly influenced adipogenesis in bone-derived MSCs. For the present study, we investigated the effect of vanadate on adipogenesis in primary rat MSCs derived from bone marrow (bmMSCs) and from the proximal end of the femur (pfMSCs). By passage 3 after isolation, both cell populations expressed the MSC cell surface markers CD90 and CD106, but not the haematopoietic marker CD45. However, although variable, expression of the fibroblast marker CD26 was higher in pfMSCs than in bmMSCs. Differentiation studies using osteogenic and adipogenic induction media (OM and AM, respectively) demonstrated that pfMSCs rapidly accumulated lipid droplets within 1 week of exposure to AM, while bmMSCs isolated from the same femur only formed lipid droplets after 3 weeks of AM treatment. Conversely, pfMSCs exposed to OM produced mineralized extracellular matrix (ECM) after 3 weeks, compared to 1 week for OM-treated bmMSCs. Vanadate (10 µM) added to AM resulted in a significant reduction in AM-induced intracellular lipid accumulation and expression of adipogenic gene markers (PPARγ2, aP2, adipsin) in both pfMSCs and bmMSCs. Pharmacological concentrations of glucocorticoids (1 µM) alone did not induce lipid accumulation in either bmMSCs or pfMSCs, but resulted in significant cell death in pfMSCs. Our findings demonstrate the existence of at least two fundamentally different MSC depots within the femur, and highlights the presence of MSCs capable of rapid adipogenesis within the proximal femur, an area prone to osteoporotic fractures. In addition, our results suggest that the increased bone marrow adiposity observed in GIO may not be solely due to direct effect of glucocorticoids on bone-derived MSCs, and that an increase in femur lipid content may also arise from increased adipogenesis in MSCs residing outside of the bone marrow niche. |
| first_indexed | 2024-12-23T05:33:49Z |
| format | Article |
| id | doaj.art-14a97e272f4d401e85068e30fb60d601 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-23T05:33:49Z |
| publishDate | 2016-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-14a97e272f4d401e85068e30fb60d6012022-12-21T17:58:23ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922016-08-01710.3389/fendo.2016.00108207329Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone.Frans Alexander Jacobs0Hanel Sadie-Van Gijsen1Mari van de Vyver2William Frank Ferris3Stellenbosch UniversityStellenbosch UniversityStellenbosch UniversityStellenbosch UniversityGlucocorticoid induced osteoporosis (GIO) is associated with an increase in bone marrow adiposity which skews the differentiation of mesenchymal stem cell (MSC) progenitors away from osteoblastogenesis and towards adipogenesis. We have previously found that vanadate, a non-specific protein tyrosine phosphatase inhibitor, prevents GIO in rats, but it was unclear whether vanadate directly influenced adipogenesis in bone-derived MSCs. For the present study, we investigated the effect of vanadate on adipogenesis in primary rat MSCs derived from bone marrow (bmMSCs) and from the proximal end of the femur (pfMSCs). By passage 3 after isolation, both cell populations expressed the MSC cell surface markers CD90 and CD106, but not the haematopoietic marker CD45. However, although variable, expression of the fibroblast marker CD26 was higher in pfMSCs than in bmMSCs. Differentiation studies using osteogenic and adipogenic induction media (OM and AM, respectively) demonstrated that pfMSCs rapidly accumulated lipid droplets within 1 week of exposure to AM, while bmMSCs isolated from the same femur only formed lipid droplets after 3 weeks of AM treatment. Conversely, pfMSCs exposed to OM produced mineralized extracellular matrix (ECM) after 3 weeks, compared to 1 week for OM-treated bmMSCs. Vanadate (10 µM) added to AM resulted in a significant reduction in AM-induced intracellular lipid accumulation and expression of adipogenic gene markers (PPARγ2, aP2, adipsin) in both pfMSCs and bmMSCs. Pharmacological concentrations of glucocorticoids (1 µM) alone did not induce lipid accumulation in either bmMSCs or pfMSCs, but resulted in significant cell death in pfMSCs. Our findings demonstrate the existence of at least two fundamentally different MSC depots within the femur, and highlights the presence of MSCs capable of rapid adipogenesis within the proximal femur, an area prone to osteoporotic fractures. In addition, our results suggest that the increased bone marrow adiposity observed in GIO may not be solely due to direct effect of glucocorticoids on bone-derived MSCs, and that an increase in femur lipid content may also arise from increased adipogenesis in MSCs residing outside of the bone marrow niche.http://journal.frontiersin.org/Journal/10.3389/fendo.2016.00108/fullAdipogenesisGlucocorticoidsMesenchymal Stem CellsBonedifferentiationosteoblast |
| spellingShingle | Frans Alexander Jacobs Hanel Sadie-Van Gijsen Mari van de Vyver William Frank Ferris Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone. Frontiers in Endocrinology Adipogenesis Glucocorticoids Mesenchymal Stem Cells Bone differentiation osteoblast |
| title | Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone. |
| title_full | Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone. |
| title_fullStr | Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone. |
| title_full_unstemmed | Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone. |
| title_short | Vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone. |
| title_sort | vanadate impedes adipogenesis in mesenchymal stem cells derived from different depots within bone |
| topic | Adipogenesis Glucocorticoids Mesenchymal Stem Cells Bone differentiation osteoblast |
| url | http://journal.frontiersin.org/Journal/10.3389/fendo.2016.00108/full |
| work_keys_str_mv | AT fransalexanderjacobs vanadateimpedesadipogenesisinmesenchymalstemcellsderivedfromdifferentdepotswithinbone AT hanelsadievangijsen vanadateimpedesadipogenesisinmesenchymalstemcellsderivedfromdifferentdepotswithinbone AT marivandevyver vanadateimpedesadipogenesisinmesenchymalstemcellsderivedfromdifferentdepotswithinbone AT williamfrankferris vanadateimpedesadipogenesisinmesenchymalstemcellsderivedfromdifferentdepotswithinbone |