Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates

Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates

Abstract An adapted strategy from the conventional 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) crosslinking method was developed to form a covalently coupled phosphoramidated single stranded DNA (ssDNA). Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) re...

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Bibliographic Details
Main Authors: Malithi P. Wickramathilaka, Bernard Y. Tao
Format: Article
Language:English
Published: BMC 2019-07-01
Series:Journal of Biological Engineering
Subjects:
EDC crosslinking
MALDI-TOF
RP-HPLC
Product yield
Reaction mechanism
Online Access:http://link.springer.com/article/10.1186/s13036-019-0191-2

Internet

http://link.springer.com/article/10.1186/s13036-019-0191-2

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