HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from <i>Alpinia zerumbet</i> (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo

Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation. Natural antioxidants may attenuate this damage. Our study aimed to investigate the chemical composition of a methanol leaf extract from <i>Alpinia zerumbet</i> and its possible antioxida...

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Main Authors: Mosad A. Ghareeb, Mansour Sobeh, Samar Rezq, Assem M. El-Shazly, Mona F. Mahmoud, Michael Wink
Format: Article
Language:English
Published: MDPI AG 2018-12-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/23/12/3238
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author Mosad A. Ghareeb
Mansour Sobeh
Samar Rezq
Assem M. El-Shazly
Mona F. Mahmoud
Michael Wink
author_facet Mosad A. Ghareeb
Mansour Sobeh
Samar Rezq
Assem M. El-Shazly
Mona F. Mahmoud
Michael Wink
author_sort Mosad A. Ghareeb
collection DOAJ
description Reactive oxygen species (ROS) have been linked to several health conditions, among them inflammation. Natural antioxidants may attenuate this damage. Our study aimed to investigate the chemical composition of a methanol leaf extract from <i>Alpinia zerumbet</i> and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects. Altogether, 37 compounds, representing benzoic and cinnamic acid derivatives and flavonoids (aglycones and glycosides), were characterized. The extract showed substantial in vitro antioxidant effects, and inhibited both cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) in vitro, with a higher selectivity towards COX-2. It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor. The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice. Furthermore, it possessed antipyretic effects against fever induced by subcutaneous injection of Brewer&#8217;s yeast in mice. Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test. These findings suggest that the leaf extract from <i>Alpinia zerumbet</i> could be a candidate for the development of a drug to treat inflammation and ROS related disorders.
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spelling doaj.art-150e70328d4949afb59fa83a270e12dd2022-12-21T23:25:22ZengMDPI AGMolecules1420-30492018-12-012312323810.3390/molecules23123238molecules23123238HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from <i>Alpinia zerumbet</i> (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In VivoMosad A. Ghareeb0Mansour Sobeh1Samar Rezq2Assem M. El-Shazly3Mona F. Mahmoud4Michael Wink5Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza 12411, EgyptInstitute of Pharmacy and Molecular Biotechnology, Heidelberg University, 44883-2462 Heidelberg, GermanyDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptInstitute of Pharmacy and Molecular Biotechnology, Heidelberg University, 44883-2462 Heidelberg, GermanyReactive oxygen species (ROS) have been linked to several health conditions, among them inflammation. Natural antioxidants may attenuate this damage. Our study aimed to investigate the chemical composition of a methanol leaf extract from <i>Alpinia zerumbet</i> and its possible antioxidant, anti-inflammatory, anti-nociceptive, and antipyretic effects. Altogether, 37 compounds, representing benzoic and cinnamic acid derivatives and flavonoids (aglycones and glycosides), were characterized. The extract showed substantial in vitro antioxidant effects, and inhibited both cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) in vitro, with a higher selectivity towards COX-2. It also inhibited 5-lipoxygenase (LOX) activity in vitro with nearly double the potency of zileuton, a reference 5-lipoxygenase (LOX) inhibitor. The extract exhibited anti-inflammatory effects against carrageenan-induced rat hind paw edema, and suppressed leukocyte infiltration into the peritoneal cavity in carrageenan-treated mice. Furthermore, it possessed antipyretic effects against fever induced by subcutaneous injection of Brewer&#8217;s yeast in mice. Additionally, the extract demonstrated both central and peripheral anti-nociceptive effects in mice, as manifested by a decrease in the count of writhing, induced with acetic acid and an increase in the latency time in the hot plate test. These findings suggest that the leaf extract from <i>Alpinia zerumbet</i> could be a candidate for the development of a drug to treat inflammation and ROS related disorders.https://www.mdpi.com/1420-3049/23/12/3238<i>Alpinia zerumbet</i>antioxidantanti-inflammatoryanalgesiaantipyreticHPLC-ESI-MS/MS
spellingShingle Mosad A. Ghareeb
Mansour Sobeh
Samar Rezq
Assem M. El-Shazly
Mona F. Mahmoud
Michael Wink
HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from <i>Alpinia zerumbet</i> (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo
Molecules
<i>Alpinia zerumbet</i>
antioxidant
anti-inflammatory
analgesia
antipyretic
HPLC-ESI-MS/MS
title HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from <i>Alpinia zerumbet</i> (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo
title_full HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from <i>Alpinia zerumbet</i> (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo
title_fullStr HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from <i>Alpinia zerumbet</i> (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo
title_full_unstemmed HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from <i>Alpinia zerumbet</i> (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo
title_short HPLC-ESI-MS/MS Profiling of Polyphenolics of a Leaf Extract from <i>Alpinia zerumbet</i> (Zingiberaceae) and Its Anti-Inflammatory, Anti-Nociceptive, and Antipyretic Activities In Vivo
title_sort hplc esi ms ms profiling of polyphenolics of a leaf extract from i alpinia zerumbet i zingiberaceae and its anti inflammatory anti nociceptive and antipyretic activities in vivo
topic <i>Alpinia zerumbet</i>
antioxidant
anti-inflammatory
analgesia
antipyretic
HPLC-ESI-MS/MS
url https://www.mdpi.com/1420-3049/23/12/3238
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