Induced hepatic stem cells are suitable for human hepatocyte production
Summary: Human hepatocytes were transfected with Sendai virus vectors (SeV) expressing OCT3/4, SOX2, KLF4, and C-MYC to produce hepatocyte-derived induced pluripotent stem cells (iPSCs). The messenger RNA (mRNA) expression of undifferentiated markers (passage 19-21) and hepatocyte-specific markers (...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222013244 |
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| author | Yoshiki Nakashima Chika Miyagi-Shiohira Issei Saitoh Masami Watanabe Masayuki Matsushita Masayoshi Tsukahara Hirofumi Noguchi |
| author_facet | Yoshiki Nakashima Chika Miyagi-Shiohira Issei Saitoh Masami Watanabe Masayuki Matsushita Masayoshi Tsukahara Hirofumi Noguchi |
| author_sort | Yoshiki Nakashima |
| collection | DOAJ |
| description | Summary: Human hepatocytes were transfected with Sendai virus vectors (SeV) expressing OCT3/4, SOX2, KLF4, and C-MYC to produce hepatocyte-derived induced pluripotent stem cells (iPSCs). The messenger RNA (mRNA) expression of undifferentiated markers (passage 19-21) and hepatocyte-specific markers (HSMs) (passage 0-20) in 48 established hepatocyte-derived iPSC-like colonies was examined. Among the 48 clones, 10 clones continuously expressed HSM mRNA (HNF1β and HNF4α) in passage 0-20. The colonies which expressed HSMs (iTS-L cells: induced tissue-specific stem cells from liver) showed a different tendency in microarray and methylation analyses to fibroblast-derived iPSCs (strain: 201B7). iTS-L cells were less likely to form teratomas in mice than iPSCs (He). The iTS-L cells were differentiated into hepatocyte-like cells more efficiently than iPSCs (He) or iPSCs (201B7). These data suggest that SeV expressing OCT3/4, SOX2, KLF4, and C-MYC induce the generation of iPSCs and iTS-L cells. |
| first_indexed | 2024-04-12T19:01:42Z |
| format | Article |
| id | doaj.art-154522eb098b4816be1c681a09bfda0f |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T19:01:42Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-154522eb098b4816be1c681a09bfda0f2022-12-22T03:20:07ZengElsevieriScience2589-00422022-10-012510105052Induced hepatic stem cells are suitable for human hepatocyte productionYoshiki Nakashima0Chika Miyagi-Shiohira1Issei Saitoh2Masami Watanabe3Masayuki Matsushita4Masayoshi Tsukahara5Hirofumi Noguchi6Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan; Kyoto University Center for iPS Cell Research and Application Foundation (CiRA Foundation), Facility for iPS Cell Therapy (FiT), Kyoto 606-8397, JapanDepartment of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDivision of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata 951-8514, JapanDepartment of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, JapanDepartment of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, JapanKyoto University Center for iPS Cell Research and Application Foundation (CiRA Foundation), Facility for iPS Cell Therapy (FiT), Kyoto 606-8397, JapanDepartment of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan; CorrespondingSummary: Human hepatocytes were transfected with Sendai virus vectors (SeV) expressing OCT3/4, SOX2, KLF4, and C-MYC to produce hepatocyte-derived induced pluripotent stem cells (iPSCs). The messenger RNA (mRNA) expression of undifferentiated markers (passage 19-21) and hepatocyte-specific markers (HSMs) (passage 0-20) in 48 established hepatocyte-derived iPSC-like colonies was examined. Among the 48 clones, 10 clones continuously expressed HSM mRNA (HNF1β and HNF4α) in passage 0-20. The colonies which expressed HSMs (iTS-L cells: induced tissue-specific stem cells from liver) showed a different tendency in microarray and methylation analyses to fibroblast-derived iPSCs (strain: 201B7). iTS-L cells were less likely to form teratomas in mice than iPSCs (He). The iTS-L cells were differentiated into hepatocyte-like cells more efficiently than iPSCs (He) or iPSCs (201B7). These data suggest that SeV expressing OCT3/4, SOX2, KLF4, and C-MYC induce the generation of iPSCs and iTS-L cells.http://www.sciencedirect.com/science/article/pii/S2589004222013244Humanbiological sciencescell biologystem cells researchdevelopmental biology |
| spellingShingle | Yoshiki Nakashima Chika Miyagi-Shiohira Issei Saitoh Masami Watanabe Masayuki Matsushita Masayoshi Tsukahara Hirofumi Noguchi Induced hepatic stem cells are suitable for human hepatocyte production iScience Human biological sciences cell biology stem cells research developmental biology |
| title | Induced hepatic stem cells are suitable for human hepatocyte production |
| title_full | Induced hepatic stem cells are suitable for human hepatocyte production |
| title_fullStr | Induced hepatic stem cells are suitable for human hepatocyte production |
| title_full_unstemmed | Induced hepatic stem cells are suitable for human hepatocyte production |
| title_short | Induced hepatic stem cells are suitable for human hepatocyte production |
| title_sort | induced hepatic stem cells are suitable for human hepatocyte production |
| topic | Human biological sciences cell biology stem cells research developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222013244 |
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