Protocol for monitoring and analyzing single nucleotide incorporation by S. cerevisiae RNA polymerases

Protocol for monitoring and analyzing single nucleotide incorporation by S. cerevisiae RNA polymerases

Summary: Here we present an optimized protocol for monitoring and analyzing single nucleotide incorporation by RNA polymerases. This protocol describes the assembly of Saccharomyces cerevisiae RNA polymerase I elongation complexes in a promoter-independent system in vitro. We describe how to collect...

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Bibliographic Details
Main Authors: Ruth Q. Jacobs, Nathan F. Bellis, Aaron L. Lucius, David A. Schneider
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Genetics
Model Organisms
Molecular Biology
Protein Biochemistry
Protein Expression and Purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001491

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http://www.sciencedirect.com/science/article/pii/S2666166723001491

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