Purification of Histidine-Tagged T4 RNA Ligase from E. coli
Here we report the construction of a histidine-tagged T4 RNA ligase expression plasmid (pRHT4). The construct, when overexpressed in BL21 (DE3) cells, allows the preparation of large quantities of T4 RNA ligase in high purity using only a single purification column. The histidine affinity tag does n...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02336st03 |