LipL41 and LigA/LigB Gene Silencing on a LipL32 Knockout <i>Leptospira interrogans</i> Reveals the Impact of Multiple Mutations on Virulence

LipL41 and LigA/LigB Gene Silencing on a LipL32 Knockout <i>Leptospira interrogans</i> Reveals the Impact of Multiple Mutations on Virulence

Leptospirosis is a global zoonosis caused by pathogenic bacteria of the genus <i>Leptospira</i>. The application of the CRISPR/Cas9 system has facilitated the generation of mutants and subsequent evaluation of phenotypes. Since DNA breaks induced by RNA-guided Cas9 nuclease are lethal to...

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Bibliographic Details
Main Authors: Luis Guilherme V. Fernandes, Bruno B. Foltran, Aline F. Teixeira, Ana Lucia Tabet Oller Nascimento
Format: Article
Language:English
Published: MDPI AG 2023-09-01
Series:Pathogens
Subjects:
<i>Leptospira</i>
knockout
knockdown
CRISPR-interference
LipL32
LipL41
Online Access:https://www.mdpi.com/2076-0817/12/10/1191

Internet

https://www.mdpi.com/2076-0817/12/10/1191

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