Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes

Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes

Abstract Background The purpose of this study was to develop a reliable DNA extraction protocol to use on individual Teladorsagia circumcincta nematode specimens to produce high quality DNA for genome sequencing and phylogenetic analysis. Pooled samples have been critical in providing the groundwork...

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Bibliographic Details
Main Authors: S. Sloan, C. J. Jenvey, D. Piedrafita, S. Preston, M. J. Stear
Format: Article
Language:English
Published: BMC 2021-05-01
Series:BMC Biotechnology
Subjects:
DNA isolation
Teladorsagia circumcincta
DNA extraction
Polymerase chain reaction
Nematode
Genome sequencing
Online Access:https://doi.org/10.1186/s12896-021-00695-6

Internet

https://doi.org/10.1186/s12896-021-00695-6

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