The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells

The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells

Abstract Background Low-temperature expression of recombinant proteins may be advantageous to support their proper folding and preserve bioactivity. The generation of expression vectors regulated under cold conditions can improve the expression of some target proteins that are difficult to express i...

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Bibliographic Details
Main Authors: Yaneth Bartolo-Aguilar, Cipriano Chávez-Cabrera, Luis Bernardo Flores-Cotera, Jesús Agustín Badillo-Corona, Carmen Oliver-Salvador, Rodolfo Marsch
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Journal of Genetic Engineering and Biotechnology
Subjects:
Cold-inducible promoter
Aggregation-prone recombinant protein
Active enzyme
Unstable gene product
Protein stability
Protein quality
Online Access:https://doi.org/10.1186/s43141-022-00455-9

Internet

https://doi.org/10.1186/s43141-022-00455-9

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