The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells
Abstract Background Low-temperature expression of recombinant proteins may be advantageous to support their proper folding and preserve bioactivity. The generation of expression vectors regulated under cold conditions can improve the expression of some target proteins that are difficult to express i...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-022-00455-9 |
| _version_ | 1797203959482417152 |
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| author | Yaneth Bartolo-Aguilar Cipriano Chávez-Cabrera Luis Bernardo Flores-Cotera Jesús Agustín Badillo-Corona Carmen Oliver-Salvador Rodolfo Marsch |
| author_facet | Yaneth Bartolo-Aguilar Cipriano Chávez-Cabrera Luis Bernardo Flores-Cotera Jesús Agustín Badillo-Corona Carmen Oliver-Salvador Rodolfo Marsch |
| author_sort | Yaneth Bartolo-Aguilar |
| collection | DOAJ |
| description | Abstract Background Low-temperature expression of recombinant proteins may be advantageous to support their proper folding and preserve bioactivity. The generation of expression vectors regulated under cold conditions can improve the expression of some target proteins that are difficult to express in different expression systems. Main body of the abstract The cspA encodes the major cold-shock protein from Escherichia coli (CspA). The promoter of cspA has been widely used to develop cold shock-inducible expression platforms in E. coli. Moreover, it is often necessary to employ expression systems other than bacteria, particularly when recombinant proteins require complex post-translational modifications. Currently, there are no commercial platforms available for expressing target genes by cold shock in eukaryotic cells. Consequently, genetic elements that respond to cold shock offer the possibility of developing novel cold-inducible expression platforms, particularly suitable for yeasts, and mammalian cells. Conclusions This review covers the importance of the cellular response to low temperatures and the prospective use of cold-sensitive promoters to direct the expression of recombinant proteins. This concept may contribute to renewing interest in applying white technologies to produce recombinant proteins that are difficult to express. Graphical Abstract |
| first_indexed | 2024-04-11T04:04:48Z |
| format | Article |
| id | doaj.art-1596248e505f41b1846b31c54abce1f6 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:27:37Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-1596248e505f41b1846b31c54abce1f62024-04-16T21:07:15ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202022-12-0120112010.1186/s43141-022-00455-9The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cellsYaneth Bartolo-Aguilar0Cipriano Chávez-Cabrera1Luis Bernardo Flores-Cotera2Jesús Agustín Badillo-Corona3Carmen Oliver-Salvador4Rodolfo Marsch5Department of Biotechnology and Bioengineering, Cinvestav-IPNColegio de Estudios Científicos y Tecnológicos del Estado de Michoacán, CECyTE Michoacán, Héroes de la Revolución S/N, Col. CentroDepartment of Biotechnology and Bioengineering, Cinvestav-IPNInstituto Politécnico Nacional-Unidad Profesional Interdisciplinaria de BiotecnologíaInstituto Politécnico Nacional-Unidad Profesional Interdisciplinaria de BiotecnologíaDepartment of Biotechnology and Bioengineering, Cinvestav-IPNAbstract Background Low-temperature expression of recombinant proteins may be advantageous to support their proper folding and preserve bioactivity. The generation of expression vectors regulated under cold conditions can improve the expression of some target proteins that are difficult to express in different expression systems. Main body of the abstract The cspA encodes the major cold-shock protein from Escherichia coli (CspA). The promoter of cspA has been widely used to develop cold shock-inducible expression platforms in E. coli. Moreover, it is often necessary to employ expression systems other than bacteria, particularly when recombinant proteins require complex post-translational modifications. Currently, there are no commercial platforms available for expressing target genes by cold shock in eukaryotic cells. Consequently, genetic elements that respond to cold shock offer the possibility of developing novel cold-inducible expression platforms, particularly suitable for yeasts, and mammalian cells. Conclusions This review covers the importance of the cellular response to low temperatures and the prospective use of cold-sensitive promoters to direct the expression of recombinant proteins. This concept may contribute to renewing interest in applying white technologies to produce recombinant proteins that are difficult to express. Graphical Abstracthttps://doi.org/10.1186/s43141-022-00455-9Cold-inducible promoterAggregation-prone recombinant proteinActive enzymeUnstable gene productProtein stabilityProtein quality |
| spellingShingle | Yaneth Bartolo-Aguilar Cipriano Chávez-Cabrera Luis Bernardo Flores-Cotera Jesús Agustín Badillo-Corona Carmen Oliver-Salvador Rodolfo Marsch The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells Journal of Genetic Engineering and Biotechnology Cold-inducible promoter Aggregation-prone recombinant protein Active enzyme Unstable gene product Protein stability Protein quality |
| title | The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells |
| title_full | The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells |
| title_fullStr | The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells |
| title_full_unstemmed | The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells |
| title_short | The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells |
| title_sort | potential of cold shock promoters for the expression of recombinant proteins in microbes and mammalian cells |
| topic | Cold-inducible promoter Aggregation-prone recombinant protein Active enzyme Unstable gene product Protein stability Protein quality |
| url | https://doi.org/10.1186/s43141-022-00455-9 |
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