DL-Methionyl–DL-Methionine/DL-Methionine Supplementation Alleviated the Adverse Effects of Dietary Low Fishmeal Levels on Growth and Intestinal Health of <i>Micropterus salmoides</i>
DL-methionyl–DL-methionine (AQUAVI® Met-Met) (Met-Met) (0.10%, 0.20%, 0.30%, and 0.40%) or DL-methionine (DL-Met) (0.10%, 0.20%, 0.30%, and 0.40%) were added to a low-fishmeal diet in an attempt to reduce fishmeal in the diet of <i>Micropterus salmoides</i> (<i>M. salmoides</i&g...
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MDPI AG
2024-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/13/3/359 |
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| author | Heng Yu Karthik Masagounder Hualiang Liang Xianping Ge Dongyu Huang Chunyu Xue Mingchun Ren Juyun He |
| author_facet | Heng Yu Karthik Masagounder Hualiang Liang Xianping Ge Dongyu Huang Chunyu Xue Mingchun Ren Juyun He |
| author_sort | Heng Yu |
| collection | DOAJ |
| description | DL-methionyl–DL-methionine (AQUAVI® Met-Met) (Met-Met) (0.10%, 0.20%, 0.30%, and 0.40%) or DL-methionine (DL-Met) (0.10%, 0.20%, 0.30%, and 0.40%) were added to a low-fishmeal diet in an attempt to reduce fishmeal in the diet of <i>Micropterus salmoides</i> (<i>M. salmoides</i>). The fish were randomly allocated into ten experimental groups (<i>n</i> = 100), each with 4 replicates of 25 fish (16.39 ± 0.01 g) each. Compared to 25% FM, 0.40% of DL-Met and 0.10% of Met-Met promoted growth, and 0.10% of Met-Met decreased FCR. Compared to 25% FM, the supplementation of Met-Met or DL-Met improved the intestinal antioxidant capacity by upregulating the NF-E2-related factor 2-mediated antioxidant factors and enzyme activities and nuclear factor kappa-B-mediated anti-inflammatory factors while downregulating the pro-inflammatory factors, thereby exerting anti-inflammatory effects. Moreover, 0.10% of the Met-Met diet affected the Firmicutes-to-Bacteroidota ratio, increased the levels of Proteobacteria, changed the composition of intestinal flora (<i>Roseburia</i>, <i>Lachnospiraceae_NK4A136_group</i>, and <i>unclassified_Oscillospiraceae</i>), and enhanced intestinal dominant bacteria (<i>Caldicoprobacter</i>, <i>Pseudogracilibacillus</i>, and <i>Parasutterella</i>), leading to improved gut health. In summary, the supplementation of DL-Met or Met-Met alleviated the adverse effect of fishmeal reduction (from 40 to 25%) on the growth performance and intestinal health of <i>M. salmoides</i>. |
| first_indexed | 2024-04-24T18:36:20Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-04-24T18:36:20Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-159acd7748e845ae864f176a97f6328f2024-03-27T13:18:41ZengMDPI AGAntioxidants2076-39212024-03-0113335910.3390/antiox13030359DL-Methionyl–DL-Methionine/DL-Methionine Supplementation Alleviated the Adverse Effects of Dietary Low Fishmeal Levels on Growth and Intestinal Health of <i>Micropterus salmoides</i>Heng Yu0Karthik Masagounder1Hualiang Liang2Xianping Ge3Dongyu Huang4Chunyu Xue5Mingchun Ren6Juyun He7Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaEvonik Operations GmbH, Hanau-Wolfgang, 63457 Hanau, GermanyWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaSchool of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, ChinaDL-methionyl–DL-methionine (AQUAVI® Met-Met) (Met-Met) (0.10%, 0.20%, 0.30%, and 0.40%) or DL-methionine (DL-Met) (0.10%, 0.20%, 0.30%, and 0.40%) were added to a low-fishmeal diet in an attempt to reduce fishmeal in the diet of <i>Micropterus salmoides</i> (<i>M. salmoides</i>). The fish were randomly allocated into ten experimental groups (<i>n</i> = 100), each with 4 replicates of 25 fish (16.39 ± 0.01 g) each. Compared to 25% FM, 0.40% of DL-Met and 0.10% of Met-Met promoted growth, and 0.10% of Met-Met decreased FCR. Compared to 25% FM, the supplementation of Met-Met or DL-Met improved the intestinal antioxidant capacity by upregulating the NF-E2-related factor 2-mediated antioxidant factors and enzyme activities and nuclear factor kappa-B-mediated anti-inflammatory factors while downregulating the pro-inflammatory factors, thereby exerting anti-inflammatory effects. Moreover, 0.10% of the Met-Met diet affected the Firmicutes-to-Bacteroidota ratio, increased the levels of Proteobacteria, changed the composition of intestinal flora (<i>Roseburia</i>, <i>Lachnospiraceae_NK4A136_group</i>, and <i>unclassified_Oscillospiraceae</i>), and enhanced intestinal dominant bacteria (<i>Caldicoprobacter</i>, <i>Pseudogracilibacillus</i>, and <i>Parasutterella</i>), leading to improved gut health. In summary, the supplementation of DL-Met or Met-Met alleviated the adverse effect of fishmeal reduction (from 40 to 25%) on the growth performance and intestinal health of <i>M. salmoides</i>.https://www.mdpi.com/2076-3921/13/3/359<i>Micropterus salmoides</i> (<i>M. salmoides</i>)Met-Metintestinal microbiotaantioxidant capacityanti-inflammatory |
| spellingShingle | Heng Yu Karthik Masagounder Hualiang Liang Xianping Ge Dongyu Huang Chunyu Xue Mingchun Ren Juyun He DL-Methionyl–DL-Methionine/DL-Methionine Supplementation Alleviated the Adverse Effects of Dietary Low Fishmeal Levels on Growth and Intestinal Health of <i>Micropterus salmoides</i> Antioxidants <i>Micropterus salmoides</i> (<i>M. salmoides</i>) Met-Met intestinal microbiota antioxidant capacity anti-inflammatory |
| title | DL-Methionyl–DL-Methionine/DL-Methionine Supplementation Alleviated the Adverse Effects of Dietary Low Fishmeal Levels on Growth and Intestinal Health of <i>Micropterus salmoides</i> |
| title_full | DL-Methionyl–DL-Methionine/DL-Methionine Supplementation Alleviated the Adverse Effects of Dietary Low Fishmeal Levels on Growth and Intestinal Health of <i>Micropterus salmoides</i> |
| title_fullStr | DL-Methionyl–DL-Methionine/DL-Methionine Supplementation Alleviated the Adverse Effects of Dietary Low Fishmeal Levels on Growth and Intestinal Health of <i>Micropterus salmoides</i> |
| title_full_unstemmed | DL-Methionyl–DL-Methionine/DL-Methionine Supplementation Alleviated the Adverse Effects of Dietary Low Fishmeal Levels on Growth and Intestinal Health of <i>Micropterus salmoides</i> |
| title_short | DL-Methionyl–DL-Methionine/DL-Methionine Supplementation Alleviated the Adverse Effects of Dietary Low Fishmeal Levels on Growth and Intestinal Health of <i>Micropterus salmoides</i> |
| title_sort | dl methionyl dl methionine dl methionine supplementation alleviated the adverse effects of dietary low fishmeal levels on growth and intestinal health of i micropterus salmoides i |
| topic | <i>Micropterus salmoides</i> (<i>M. salmoides</i>) Met-Met intestinal microbiota antioxidant capacity anti-inflammatory |
| url | https://www.mdpi.com/2076-3921/13/3/359 |
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