A DNA-Free Editing Platform for Genetic Screens in Soybean via CRISPR/Cas9 Ribonucleoprotein Delivery

A DNA-Free Editing Platform for Genetic Screens in Soybean via CRISPR/Cas9 Ribonucleoprotein Delivery

CRISPR/Cas9-based ribonucleoprotein (RNP)-mediated system has the property of minimizing the effects related to the unwanted introduction of vector DNA and random integration of recombinant DNA. Here, we describe a platform based on the direct delivery of Cas9 RNPs to soybean protoplasts for genetic...

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Bibliographic Details
Main Authors: Saminathan Subburaj, Caroline Bedin Zanatta, Jennifer A. L. Nunn, Aline Martins Hoepers, Rubens Onofre Nodari, Sarah Zanon Agapito-Tenfen
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-07-01
Series:Frontiers in Plant Science
Subjects:
genetically modified organism
gene editing
mutagenesis
transgenesis
target deep sequencing
breeding
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2022.939997/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2022.939997/full

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