The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds
The aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality traits in sheep. The FABP4 cDNA was cloned by RT-PCR method, and the sequence analysis showed that the op...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2011-01-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201101-0001_the-fabp4-gene-polymorphism-is-associated-with-meat-tenderness-in-three-chinese-native-sheep-breeds.php |
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| author | Q.L. Xu G.W. Tang Q.L. Zhang Y.K. Huang Y.X. Liu K. Quan K.Y. Zhu C.X. Zhang |
| author_facet | Q.L. Xu G.W. Tang Q.L. Zhang Y.K. Huang Y.X. Liu K. Quan K.Y. Zhu C.X. Zhang |
| author_sort | Q.L. Xu |
| collection | DOAJ |
| description | The aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality traits in sheep. The FABP4 cDNA was cloned by RT-PCR method, and the sequence analysis showed that the open reading frame of sheep FABP4 is 399 bp and codes 132 amino acids. A mutation (A/G) detected in intron 1 of FABP4 gene was studied in 286 lambs of three Chinese native sheep breeds by PCR-SSCP procedure. Significant statistical association results revealed that AA genotype conferred higher tenderness (P < 0.05), muscle marbling score (P < 0.05) and intramuscular fat content (IMF; P < 0.05). Thus we suggested that the genotype AA could be regarded as a molecular marker for LT meat tenderness and IMF content in sheep. |
| first_indexed | 2024-04-10T08:26:52Z |
| format | Article |
| id | doaj.art-15ae631c247947bc8c717a49d2bf3f69 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:26:52Z |
| publishDate | 2011-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-15ae631c247947bc8c717a49d2bf3f692023-02-23T03:32:53ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092011-01-015611610.17221/231/2009-CJAScjs-201101-0001The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breedsQ.L. Xu0G.W. Tang1Q.L. Zhang2Y.K. Huang3Y.X. Liu4K. Quan5K.Y. Zhu6C.X. Zhang7Department of Animal Science and Technology, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan, P.R. ChinaDepartment of Animal Science and Technology, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan, P.R. ChinaDepartment of Animal Science and Technology, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan, P.R. ChinaDepartment of Animal Science and Technology, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan, P.R. ChinaDepartment of Animal Science and Technology, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan, P.R. ChinaDepartment of Animal Science and Technology, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan, P.R. ChinaDepartment of Animal Science and Technology, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan, P.R. ChinaDepartment of Animal Science and Technology, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan, P.R. ChinaThe aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality traits in sheep. The FABP4 cDNA was cloned by RT-PCR method, and the sequence analysis showed that the open reading frame of sheep FABP4 is 399 bp and codes 132 amino acids. A mutation (A/G) detected in intron 1 of FABP4 gene was studied in 286 lambs of three Chinese native sheep breeds by PCR-SSCP procedure. Significant statistical association results revealed that AA genotype conferred higher tenderness (P < 0.05), muscle marbling score (P < 0.05) and intramuscular fat content (IMF; P < 0.05). Thus we suggested that the genotype AA could be regarded as a molecular marker for LT meat tenderness and IMF content in sheep.https://cjas.agriculturejournals.cz/artkey/cjs-201101-0001_the-fabp4-gene-polymorphism-is-associated-with-meat-tenderness-in-three-chinese-native-sheep-breeds.phpsheepfabp4 genepolymorphismintramuscular fat |
| spellingShingle | Q.L. Xu G.W. Tang Q.L. Zhang Y.K. Huang Y.X. Liu K. Quan K.Y. Zhu C.X. Zhang The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds Czech Journal of Animal Science sheep fabp4 gene polymorphism intramuscular fat |
| title | The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds |
| title_full | The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds |
| title_fullStr | The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds |
| title_full_unstemmed | The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds |
| title_short | The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds |
| title_sort | fabp4 gene polymorphism is associated with meat tenderness in three chinese native sheep breeds |
| topic | sheep fabp4 gene polymorphism intramuscular fat |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201101-0001_the-fabp4-gene-polymorphism-is-associated-with-meat-tenderness-in-three-chinese-native-sheep-breeds.php |
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