Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays
Two flavonoids, rutin and hesperidin, were investigated in vitro for anticoagulant activity through coagulation tests: activated partial thromboplastin time (aPTT), prothrombin time (PT) and thrombin time (TT). Only an ethanolic solution of rutin at the concentration of 830 µM prolonged aPTT, while...
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-02-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/16/2/1378/ |
| _version_ | 1818229930345889792 |
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| author | Vesna Kuntić Zorica Vujić Ivana Filipović |
| author_facet | Vesna Kuntić Zorica Vujić Ivana Filipović |
| author_sort | Vesna Kuntić |
| collection | DOAJ |
| description | Two flavonoids, rutin and hesperidin, were investigated in vitro for anticoagulant activity through coagulation tests: activated partial thromboplastin time (aPTT), prothrombin time (PT) and thrombin time (TT). Only an ethanolic solution of rutin at the concentration of 830 µM prolonged aPTT, while TT and PT were unaffected. In order to evaluate whether the prolongation of aPTT was due to the decrease of coagulation factors, the experiment with deficient plasma was performed, showing the effects on factors VIII and IX. Since pharmacological activity of flavonoids is believed to increase when they are coordinated with metal ions, complexes of these flavonoids with Al(III) and Cu(II) ions were also tested. The results showed that complexes significantly prolonged aPTT and had no effects on PT and TT. Assay with deficient plasma (plasma having the investigated factor at less then 1%) revealed that complexes could bind to the coagulation factors, what may lead to a non-specific inhibition and aPTT prolongation. An effort was made to correlate stability of complexes with their anticoagulant properties. |
| first_indexed | 2024-12-12T10:26:25Z |
| format | Article |
| id | doaj.art-15b18e4f99344e7a8217aa8d670f8771 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T10:26:25Z |
| publishDate | 2011-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-15b18e4f99344e7a8217aa8d670f87712022-12-22T00:27:26ZengMDPI AGMolecules1420-30492011-02-011621378138810.3390/molecules16021378Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation AssaysVesna KuntićZorica VujićIvana FilipovićTwo flavonoids, rutin and hesperidin, were investigated in vitro for anticoagulant activity through coagulation tests: activated partial thromboplastin time (aPTT), prothrombin time (PT) and thrombin time (TT). Only an ethanolic solution of rutin at the concentration of 830 µM prolonged aPTT, while TT and PT were unaffected. In order to evaluate whether the prolongation of aPTT was due to the decrease of coagulation factors, the experiment with deficient plasma was performed, showing the effects on factors VIII and IX. Since pharmacological activity of flavonoids is believed to increase when they are coordinated with metal ions, complexes of these flavonoids with Al(III) and Cu(II) ions were also tested. The results showed that complexes significantly prolonged aPTT and had no effects on PT and TT. Assay with deficient plasma (plasma having the investigated factor at less then 1%) revealed that complexes could bind to the coagulation factors, what may lead to a non-specific inhibition and aPTT prolongation. An effort was made to correlate stability of complexes with their anticoagulant properties.http://www.mdpi.com/1420-3049/16/2/1378/rutinhesperidinflavonoid-metal complexesanticoagulation actionaPTTPTTT |
| spellingShingle | Vesna Kuntić Zorica Vujić Ivana Filipović Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays Molecules rutin hesperidin flavonoid-metal complexes anticoagulation action aPTT PT TT |
| title | Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays |
| title_full | Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays |
| title_fullStr | Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays |
| title_full_unstemmed | Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays |
| title_short | Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays |
| title_sort | effects of rutin and hesperidin and their al iii and cu ii complexes on in vitro plasma coagulation assays |
| topic | rutin hesperidin flavonoid-metal complexes anticoagulation action aPTT PT TT |
| url | http://www.mdpi.com/1420-3049/16/2/1378/ |
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