Revisiting the fundamentals of p-nitrophenol analysis for its application in the quantification of lipases activity. A graphical update.
p-Nitrophenol (pNP) is a widely used compound for analytical determinations of several esterases (EC. 3.1.1.X), including lipases (E.C. 3.1.1.3). Most enzymatic measurements employ pNP derivatives such as esters, which are broken down by enzymatic hydrolysis, releasing pNP that is quantified by its...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Spanish |
| Published: |
Universidad Nacional, Costa Rica
2020-07-01
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| Series: | Uniciencia |
| Subjects: | |
| Online Access: | https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/13584 |