Protocol for microinjection of rapamycin into the zebrafish habenula

Summary: Mechanistic target of rapamycin complex 1 (mTorC1) activity plays a crucial role in brain development. Here, we present an approach for rapamycin microinjection into the habenula of larval zebrafish to achieve localized inhibition of the mTorC1 pathway and explore the role of mTorC1 in habe...

Full description

Bibliographic Details
Main Authors: Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007317