Dengue viral RNA levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status.
Infection with dengue viruses (DENV) causes a wide range of manifestations from asymptomatic infection to a febrile illness called dengue fever (DF), to dengue hemorrhagic fever (DHF). The in vivo targets of DENV and the relation between the viral burden in these cells and disease severity are not k...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3526575?pdf=render |
| _version_ | 1828199224564514816 |
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| author | Anon Srikiatkhachorn Sineewanlaya Wichit Robert V Gibbons Sharone Green Daniel H Libraty Timothy P Endy Francis A Ennis Siripen Kalayanarooj Alan L Rothman |
| author_facet | Anon Srikiatkhachorn Sineewanlaya Wichit Robert V Gibbons Sharone Green Daniel H Libraty Timothy P Endy Francis A Ennis Siripen Kalayanarooj Alan L Rothman |
| author_sort | Anon Srikiatkhachorn |
| collection | DOAJ |
| description | Infection with dengue viruses (DENV) causes a wide range of manifestations from asymptomatic infection to a febrile illness called dengue fever (DF), to dengue hemorrhagic fever (DHF). The in vivo targets of DENV and the relation between the viral burden in these cells and disease severity are not known.The levels of positive and negative strand viral RNA in peripheral blood monocytes, T/NK cells, and B cells and in plasma of DF and DHF cases were measured by quantitative RT-PCR.Positive strand viral RNA was detected in monocytes, T/NK cells and B cells with the highest amounts found in B cells. Viral RNA levels in CD14+ cells and plasma were significantly higher in DHF compared to DF, and in cases with a secondary infection compared to those undergoing a primary infection. The distribution of viral RNA among cell subpopulations was similar in DF and DHF cases. Small amounts of negative strand RNA were found in a few cases only. The severity of plasma leakage correlated with viral RNA levels in plasma and in CD14+ cells.B cells were the principal cells containing DENV RNA in peripheral blood, but overall there was little active DENV RNA replication detectable in peripheral blood mononuclear cells (PBMC). Secondary infection and DHF were associated with higher viral burden in PBMC populations, especially CD14+ monocytes, suggesting that viral infection of these cells may be involved in disease pathogenesis. |
| first_indexed | 2024-04-12T10:51:49Z |
| format | Article |
| id | doaj.art-161cf334486649109a4390ac4a1514ba |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T10:51:49Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-161cf334486649109a4390ac4a1514ba2022-12-22T03:36:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5133510.1371/journal.pone.0051335Dengue viral RNA levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status.Anon SrikiatkhachornSineewanlaya WichitRobert V GibbonsSharone GreenDaniel H LibratyTimothy P EndyFrancis A EnnisSiripen KalayanaroojAlan L RothmanInfection with dengue viruses (DENV) causes a wide range of manifestations from asymptomatic infection to a febrile illness called dengue fever (DF), to dengue hemorrhagic fever (DHF). The in vivo targets of DENV and the relation between the viral burden in these cells and disease severity are not known.The levels of positive and negative strand viral RNA in peripheral blood monocytes, T/NK cells, and B cells and in plasma of DF and DHF cases were measured by quantitative RT-PCR.Positive strand viral RNA was detected in monocytes, T/NK cells and B cells with the highest amounts found in B cells. Viral RNA levels in CD14+ cells and plasma were significantly higher in DHF compared to DF, and in cases with a secondary infection compared to those undergoing a primary infection. The distribution of viral RNA among cell subpopulations was similar in DF and DHF cases. Small amounts of negative strand RNA were found in a few cases only. The severity of plasma leakage correlated with viral RNA levels in plasma and in CD14+ cells.B cells were the principal cells containing DENV RNA in peripheral blood, but overall there was little active DENV RNA replication detectable in peripheral blood mononuclear cells (PBMC). Secondary infection and DHF were associated with higher viral burden in PBMC populations, especially CD14+ monocytes, suggesting that viral infection of these cells may be involved in disease pathogenesis.http://europepmc.org/articles/PMC3526575?pdf=render |
| spellingShingle | Anon Srikiatkhachorn Sineewanlaya Wichit Robert V Gibbons Sharone Green Daniel H Libraty Timothy P Endy Francis A Ennis Siripen Kalayanarooj Alan L Rothman Dengue viral RNA levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status. PLoS ONE |
| title | Dengue viral RNA levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status. |
| title_full | Dengue viral RNA levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status. |
| title_fullStr | Dengue viral RNA levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status. |
| title_full_unstemmed | Dengue viral RNA levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status. |
| title_short | Dengue viral RNA levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status. |
| title_sort | dengue viral rna levels in peripheral blood mononuclear cells are associated with disease severity and preexisting dengue immune status |
| url | http://europepmc.org/articles/PMC3526575?pdf=render |
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