Roles of Bcl-2 and caspase-9 and -3 in CD30-induced human eosinophil apoptosis
Background/purpose: Activation of cell surface CD30 by immobilized anti-CD30 monoclonal antibodies (mAb) induces strong apoptosis in human eosinophils. This anti-CD30 mAb-induced eosinophil apoptosis is inhibited by the addition of inhibitors of p38, ERK1/2 mitogen-activated protein kinases, and pho...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-04-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118215007732 |
| _version_ | 1819230574698561536 |
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| author | Hye Jin Lee Eun-Kyoung Lee Young Eun Seo Youn Ho Shin Hwan Soo Kim Yoon Hong Chun Jong-Seo Yoon Hyun Hee Kim Man Yong Han Chang-Keun Kim Kyu-Earn Kim Young Yull Koh Jin Tack Kim |
| author_facet | Hye Jin Lee Eun-Kyoung Lee Young Eun Seo Youn Ho Shin Hwan Soo Kim Yoon Hong Chun Jong-Seo Yoon Hyun Hee Kim Man Yong Han Chang-Keun Kim Kyu-Earn Kim Young Yull Koh Jin Tack Kim |
| author_sort | Hye Jin Lee |
| collection | DOAJ |
| description | Background/purpose: Activation of cell surface CD30 by immobilized anti-CD30 monoclonal antibodies (mAb) induces strong apoptosis in human eosinophils. This anti-CD30 mAb-induced eosinophil apoptosis is inhibited by the addition of inhibitors of p38, ERK1/2 mitogen-activated protein kinases, and phosphatidylinositol 3-kinase. However, there is little data investigating the role of Bcl-2 and caspases in eosinophil apoptosis induced by anti-CD30 mAb. We sought to determine whether anti-CD30 mAb induces human eosinophil apoptosis via Bcl-2 and caspase pathways.
Methods: Peripheral blood was drawn from 37 healthy volunteers. The CD30 expression on eosinophils was measured at various time points. Eosinophils were then cultured in plates precoated with anti-CD30 mAb (clone Ber-H8), isotype control immunoglobulin G1, interleukin (IL)-5, or dexamethasone. Western blot analysis was performed to determine the expression of Bcl-2, procaspase-8, -9, and -3, and caspase-8, -9, and -3 after cross-linking of CD30. Human eosinophils were also cultured in plates precoated with anti-CD30 mAb (clone Ber-H8) in the presence or absence of caspase-9 or -3 inhibitors. Eosinophil apoptosis was assessed using flow cytometry.
Results: The addition of anti-CD30 mAb significantly increased eosinophil apoptosis compared with controls. In western blot analysis, the addition of anti-CD30 mAb significantly decreased the expression of Bcl-2 and procaspase-9 and -3 and increased the expression of caspase-9 and -3. The addition of caspase-9 or -3 inhibitors decreased anti-CD30 mAb-induced human eosinophil apoptosis. Procaspase-8 or caspase-8 expression was not changed in response to various stimuli.
Conclusion: Anti-CD30 mAb-induced human eosinophil apoptosis is likely to be mediated through Bcl-2 and caspase-9 and -3. |
| first_indexed | 2024-12-23T11:31:14Z |
| format | Article |
| id | doaj.art-1624d0c4fda1437993db8b4b03c7cf02 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-23T11:31:14Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-1624d0c4fda1437993db8b4b03c7cf022022-12-21T17:48:47ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822017-04-0150214515210.1016/j.jmii.2015.05.017Roles of Bcl-2 and caspase-9 and -3 in CD30-induced human eosinophil apoptosisHye Jin Lee0Eun-Kyoung Lee1Young Eun Seo2Youn Ho Shin3Hwan Soo Kim4Yoon Hong Chun5Jong-Seo Yoon6Hyun Hee Kim7Man Yong Han8Chang-Keun Kim9Kyu-Earn Kim10Young Yull Koh11Jin Tack Kim12Department of Pediatrics, School of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Pediatrics, School of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Pediatrics, School of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Medicine, The Graduate School, Yonsei University, Seoul, Republic of KoreaDepartment of Pediatrics, School of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Pediatrics, School of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Pediatrics, School of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Pediatrics, School of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Pediatrics, CHA University School of Medicine, Seoul, Republic of KoreaAsthma and Allergy Center, Department of Pediatrics, Inje University Sanggye Paik Hospital, Seoul, Republic of KoreaDepartment of Pediatrics, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Pediatrics, Seoul National University Hospital, Seoul, Republic of KoreaDepartment of Pediatrics, School of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaBackground/purpose: Activation of cell surface CD30 by immobilized anti-CD30 monoclonal antibodies (mAb) induces strong apoptosis in human eosinophils. This anti-CD30 mAb-induced eosinophil apoptosis is inhibited by the addition of inhibitors of p38, ERK1/2 mitogen-activated protein kinases, and phosphatidylinositol 3-kinase. However, there is little data investigating the role of Bcl-2 and caspases in eosinophil apoptosis induced by anti-CD30 mAb. We sought to determine whether anti-CD30 mAb induces human eosinophil apoptosis via Bcl-2 and caspase pathways. Methods: Peripheral blood was drawn from 37 healthy volunteers. The CD30 expression on eosinophils was measured at various time points. Eosinophils were then cultured in plates precoated with anti-CD30 mAb (clone Ber-H8), isotype control immunoglobulin G1, interleukin (IL)-5, or dexamethasone. Western blot analysis was performed to determine the expression of Bcl-2, procaspase-8, -9, and -3, and caspase-8, -9, and -3 after cross-linking of CD30. Human eosinophils were also cultured in plates precoated with anti-CD30 mAb (clone Ber-H8) in the presence or absence of caspase-9 or -3 inhibitors. Eosinophil apoptosis was assessed using flow cytometry. Results: The addition of anti-CD30 mAb significantly increased eosinophil apoptosis compared with controls. In western blot analysis, the addition of anti-CD30 mAb significantly decreased the expression of Bcl-2 and procaspase-9 and -3 and increased the expression of caspase-9 and -3. The addition of caspase-9 or -3 inhibitors decreased anti-CD30 mAb-induced human eosinophil apoptosis. Procaspase-8 or caspase-8 expression was not changed in response to various stimuli. Conclusion: Anti-CD30 mAb-induced human eosinophil apoptosis is likely to be mediated through Bcl-2 and caspase-9 and -3.http://www.sciencedirect.com/science/article/pii/S1684118215007732apoptosisBcl-2caspaseseosinophils |
| spellingShingle | Hye Jin Lee Eun-Kyoung Lee Young Eun Seo Youn Ho Shin Hwan Soo Kim Yoon Hong Chun Jong-Seo Yoon Hyun Hee Kim Man Yong Han Chang-Keun Kim Kyu-Earn Kim Young Yull Koh Jin Tack Kim Roles of Bcl-2 and caspase-9 and -3 in CD30-induced human eosinophil apoptosis Journal of Microbiology, Immunology and Infection apoptosis Bcl-2 caspases eosinophils |
| title | Roles of Bcl-2 and caspase-9 and -3 in CD30-induced human eosinophil apoptosis |
| title_full | Roles of Bcl-2 and caspase-9 and -3 in CD30-induced human eosinophil apoptosis |
| title_fullStr | Roles of Bcl-2 and caspase-9 and -3 in CD30-induced human eosinophil apoptosis |
| title_full_unstemmed | Roles of Bcl-2 and caspase-9 and -3 in CD30-induced human eosinophil apoptosis |
| title_short | Roles of Bcl-2 and caspase-9 and -3 in CD30-induced human eosinophil apoptosis |
| title_sort | roles of bcl 2 and caspase 9 and 3 in cd30 induced human eosinophil apoptosis |
| topic | apoptosis Bcl-2 caspases eosinophils |
| url | http://www.sciencedirect.com/science/article/pii/S1684118215007732 |
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