Preparation of B2M and PD-1 Efficiently Double Genes Knock-out Primary Human T Lymphocytes by Cas9 RNP Technology

Preparation of B2M and PD-1 Efficiently Double Genes Knock-out Primary Human T Lymphocytes by Cas9 RNP Technology

Objective To observe the efficiency of Cas9 RNP technology for the preparation of B2M and PD-1 double genes knock-out primary human T lymphocytes (hereinafter referred to as T cells). Methods Single-guide RNA (sgRNA) was designed and cloned into vector plasmids. The effective sgRNAs were screened ou...

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Bibliographic Details
Main Authors: XU Ya, FU Yang, ZHU Shicong, ZHANG Bicheng
Format: Article
Language:zho
Published: Magazine House of Cancer Research on Prevention and Treatment 2020-06-01
Series:Zhongliu Fangzhi Yanjiu
Subjects:
cas9
ribonucleoproteins
pd-1
beta-2 microglobulin
t lymphocytes
Online Access:http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.1603.htm

Internet

http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.1603.htm

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