Preparation of B2M and PD-1 Efficiently Double Genes Knock-out Primary Human T Lymphocytes by Cas9 RNP Technology
Objective To observe the efficiency of Cas9 RNP technology for the preparation of B2M and PD-1 double genes knock-out primary human T lymphocytes (hereinafter referred to as T cells). Methods Single-guide RNA (sgRNA) was designed and cloned into vector plasmids. The effective sgRNAs were screened ou...
Main Authors: | XU Ya, FU Yang, ZHU Shicong, ZHANG Bicheng |
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Format: | Article |
Language: | zho |
Published: |
Magazine House of Cancer Research on Prevention and Treatment
2020-06-01
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Series: | Zhongliu Fangzhi Yanjiu |
Subjects: | |
Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.1603.htm |
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