<b>Optimization of olive oil hydrolysis process using immobilized Lipase from <i>Burkholderia cepacia</i> sp. in Polyurethane
The aim of this study was to achieve the best conditions for the olive oil hydrolysis process at optimal pH and temperature using Burkholderia cepacia lipase immobilized in situ in rigid polyurethane support. The influences of the temperature (13.85 to 56.5ºC) and pH (4.18 to 9.82) were evaluated b...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Maringá
2017-09-01
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| Series: | Acta Scientiarum: Technology |
| Subjects: | |
| Online Access: | http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/30770 |
| _version_ | 1811328633901416448 |
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| author | Nádia Ligianara Dewes Nyari Alessandro Rogério Paulazzi Clarice Steffens Marcelo Mignoni Jamile Zeni Rogério Marcos Dallago |
| author_facet | Nádia Ligianara Dewes Nyari Alessandro Rogério Paulazzi Clarice Steffens Marcelo Mignoni Jamile Zeni Rogério Marcos Dallago |
| author_sort | Nádia Ligianara Dewes Nyari |
| collection | DOAJ |
| description | The aim of this study was to achieve the best conditions for the olive oil hydrolysis process at optimal pH and temperature using Burkholderia cepacia lipase immobilized in situ in rigid polyurethane support. The influences of the temperature (13.85 to 56.5ºC) and pH (4.18 to 9.82) were evaluated by a central composite rotational experimental design 22. The operational stability and storage conditions were also studied. The olive oil hydrolysis process was optimized in pH 7.0, at 40°C and 15 min of reaction, with 66 and 93 U g-1 of hydrolysis activity in free and immobilized lipase, respectively, with > 700% yield. The immobilized remained stable for up to 40 days of storage at temperatures of 60oC, and for 100 days from 4 to 25°C. The operational stability of the immobilized was 6 continuous cycles. In this way, immobilization showed to be a promising alternative for its application in olive oil hydrolysis, having storage stability and reuse capability. |
| first_indexed | 2024-04-13T15:29:37Z |
| format | Article |
| id | doaj.art-163dc0e02c3a4ed7adbc848e93ee21a3 |
| institution | Directory Open Access Journal |
| issn | 1806-2563 1807-8664 |
| language | English |
| last_indexed | 2024-04-13T15:29:37Z |
| publishDate | 2017-09-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Technology |
| spelling | doaj.art-163dc0e02c3a4ed7adbc848e93ee21a32022-12-22T02:41:25ZengUniversidade Estadual de MaringáActa Scientiarum: Technology1806-25631807-86642017-09-0139438539310.4025/actascitechnol.v39i4.3077016338<b>Optimization of olive oil hydrolysis process using immobilized Lipase from <i>Burkholderia cepacia</i> sp. in PolyurethaneNádia Ligianara Dewes Nyari0Alessandro Rogério Paulazzi1Clarice Steffens2Marcelo Mignoni3Jamile Zeni4Rogério Marcos Dallago5Universidade Regional do Alto Uruguai e das MissõesUniversidade Regional do Alto Uruguai e das MissõesUniversidade Regional do Alto Uruguai e das MissõesUniversidade Regional do Alto Uruguai e das MissõesUniversidade Regional do Alto Uruguai e das MissõesUniversidade Regional do Alto Uruguai e das MissõesThe aim of this study was to achieve the best conditions for the olive oil hydrolysis process at optimal pH and temperature using Burkholderia cepacia lipase immobilized in situ in rigid polyurethane support. The influences of the temperature (13.85 to 56.5ºC) and pH (4.18 to 9.82) were evaluated by a central composite rotational experimental design 22. The operational stability and storage conditions were also studied. The olive oil hydrolysis process was optimized in pH 7.0, at 40°C and 15 min of reaction, with 66 and 93 U g-1 of hydrolysis activity in free and immobilized lipase, respectively, with > 700% yield. The immobilized remained stable for up to 40 days of storage at temperatures of 60oC, and for 100 days from 4 to 25°C. The operational stability of the immobilized was 6 continuous cycles. In this way, immobilization showed to be a promising alternative for its application in olive oil hydrolysis, having storage stability and reuse capability.http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/30770biocatalysislipasein situ immobilizationpolyurethanehydrolysis. |
| spellingShingle | Nádia Ligianara Dewes Nyari Alessandro Rogério Paulazzi Clarice Steffens Marcelo Mignoni Jamile Zeni Rogério Marcos Dallago <b>Optimization of olive oil hydrolysis process using immobilized Lipase from <i>Burkholderia cepacia</i> sp. in Polyurethane Acta Scientiarum: Technology biocatalysis lipase in situ immobilization polyurethane hydrolysis. |
| title | <b>Optimization of olive oil hydrolysis process using immobilized Lipase from <i>Burkholderia cepacia</i> sp. in Polyurethane |
| title_full | <b>Optimization of olive oil hydrolysis process using immobilized Lipase from <i>Burkholderia cepacia</i> sp. in Polyurethane |
| title_fullStr | <b>Optimization of olive oil hydrolysis process using immobilized Lipase from <i>Burkholderia cepacia</i> sp. in Polyurethane |
| title_full_unstemmed | <b>Optimization of olive oil hydrolysis process using immobilized Lipase from <i>Burkholderia cepacia</i> sp. in Polyurethane |
| title_short | <b>Optimization of olive oil hydrolysis process using immobilized Lipase from <i>Burkholderia cepacia</i> sp. in Polyurethane |
| title_sort | b optimization of olive oil hydrolysis process using immobilized lipase from i burkholderia cepacia i sp in polyurethane |
| topic | biocatalysis lipase in situ immobilization polyurethane hydrolysis. |
| url | http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/30770 |
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