Determination of Chitin Content in Insects: An Alternate Method Based on Calcofluor Staining

Chitin is an aminopolysaccharide present in yeast cells and arthropod cuticle and is one of the most abundant biopolymers. The conventional methods for the quantitation of chitin content in biological samples are based on its hydrolysis (acid or enzymatic), and the assessment of the byproduct, gluco...

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Bibliographic Details
Main Authors: Bianca Santos Henriques, Eloi Souza Garcia, Patricia Azambuja, Fernando Ariel Genta
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-02-01
Series:Frontiers in Physiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fphys.2020.00117/full