Calculating the force-dependent unbinding rate of biological macromolecular bonds from force-ramp optical trapping assays
Abstract The non-covalent biological bonds that constitute protein–protein or protein–ligand interactions play crucial roles in many cellular functions, including mitosis, motility, and cell–cell adhesion. The effect of external force ( $$F$$ F ) on the unbinding rate ( $${k}_{\text{off}}\left(F\rig...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-03690-1 |