Understanding the Effect of Ozone on <i>Listeria monocytogenes</i> and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach
The occurrence of <i>Listeria monocytogenes</i> on Gorgonzola cheese surface was reported by many authors, with risks arising from the translocation of the pathogen inside the product during cutting procedures. Among the novel antimicrobial strategies, ozone may represent a useful tool a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/17/2640 |
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| author | Felice Panebianco Selene Rubiola Chiara Buttieri Pierluigi Aldo Di Ciccio Francesco Chiesa Tiziana Civera |
| author_facet | Felice Panebianco Selene Rubiola Chiara Buttieri Pierluigi Aldo Di Ciccio Francesco Chiesa Tiziana Civera |
| author_sort | Felice Panebianco |
| collection | DOAJ |
| description | The occurrence of <i>Listeria monocytogenes</i> on Gorgonzola cheese surface was reported by many authors, with risks arising from the translocation of the pathogen inside the product during cutting procedures. Among the novel antimicrobial strategies, ozone may represent a useful tool against <i>L. monocytogenes</i> contamination on Gorgonzola cheese rind. In this study, the effect of gaseous ozone (2 and 4 ppm for 10 min) on <i>L. monocytogenes</i> and resident microbiota of Gorgonzola cheese rind stored at 4 °C for 63 days was evaluated. A culturomic approach, based on the use of six media and identification of colonies by MALDI-TOF MS, was used to analyse variations of resident populations. The decrease of <i>L. monocytogenes</i> was less pronounced in ozonised rinds with final loads of ~1 log CFU/g higher than controls. This behaviour coincided with a lower maximum population density of lactobacilli in treated samples at day 28. No significant differences were detected for the other microbial determinations and resident microbiota composition among treated and control samples. The dominant genera were <i>Candida</i>, <i>Carnobacterium</i>, <i>Staphylococcus</i>, <i>Penicillium</i>, <i>Saccharomyces</i>, <i>Aerococcus</i>, <i>Yarrowia</i>, and <i>Enterococcus</i>. Based on our results, ozone was ineffective against <i>L. monocytogenes</i> contamination on Gorgonzola rinds. The higher final <i>L. monocytogenes</i> loads in treated samples could be associated with a suppressive effect of ozone on lactobacilli, since these are antagonists of <i>L. monocytogenes</i>. Our outcomes suggest the potential use of culturomics to study the ecosystems of complex matrices, such as the surface of mould and blue-veined cheeses. |
| first_indexed | 2024-03-10T01:48:45Z |
| format | Article |
| id | doaj.art-16692cf503c64744abb80c67bf4ed5f3 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T01:48:45Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-16692cf503c64744abb80c67bf4ed5f32023-11-23T13:08:46ZengMDPI AGFoods2304-81582022-08-011117264010.3390/foods11172640Understanding the Effect of Ozone on <i>Listeria monocytogenes</i> and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic ApproachFelice Panebianco0Selene Rubiola1Chiara Buttieri2Pierluigi Aldo Di Ciccio3Francesco Chiesa4Tiziana Civera5Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, ItalyThe occurrence of <i>Listeria monocytogenes</i> on Gorgonzola cheese surface was reported by many authors, with risks arising from the translocation of the pathogen inside the product during cutting procedures. Among the novel antimicrobial strategies, ozone may represent a useful tool against <i>L. monocytogenes</i> contamination on Gorgonzola cheese rind. In this study, the effect of gaseous ozone (2 and 4 ppm for 10 min) on <i>L. monocytogenes</i> and resident microbiota of Gorgonzola cheese rind stored at 4 °C for 63 days was evaluated. A culturomic approach, based on the use of six media and identification of colonies by MALDI-TOF MS, was used to analyse variations of resident populations. The decrease of <i>L. monocytogenes</i> was less pronounced in ozonised rinds with final loads of ~1 log CFU/g higher than controls. This behaviour coincided with a lower maximum population density of lactobacilli in treated samples at day 28. No significant differences were detected for the other microbial determinations and resident microbiota composition among treated and control samples. The dominant genera were <i>Candida</i>, <i>Carnobacterium</i>, <i>Staphylococcus</i>, <i>Penicillium</i>, <i>Saccharomyces</i>, <i>Aerococcus</i>, <i>Yarrowia</i>, and <i>Enterococcus</i>. Based on our results, ozone was ineffective against <i>L. monocytogenes</i> contamination on Gorgonzola rinds. The higher final <i>L. monocytogenes</i> loads in treated samples could be associated with a suppressive effect of ozone on lactobacilli, since these are antagonists of <i>L. monocytogenes</i>. Our outcomes suggest the potential use of culturomics to study the ecosystems of complex matrices, such as the surface of mould and blue-veined cheeses.https://www.mdpi.com/2304-8158/11/17/2640cheeseculturomicsfood safety<i>Listeria monocytogenes</i>MALDI-TOF MSozone |
| spellingShingle | Felice Panebianco Selene Rubiola Chiara Buttieri Pierluigi Aldo Di Ciccio Francesco Chiesa Tiziana Civera Understanding the Effect of Ozone on <i>Listeria monocytogenes</i> and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach Foods cheese culturomics food safety <i>Listeria monocytogenes</i> MALDI-TOF MS ozone |
| title | Understanding the Effect of Ozone on <i>Listeria monocytogenes</i> and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach |
| title_full | Understanding the Effect of Ozone on <i>Listeria monocytogenes</i> and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach |
| title_fullStr | Understanding the Effect of Ozone on <i>Listeria monocytogenes</i> and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach |
| title_full_unstemmed | Understanding the Effect of Ozone on <i>Listeria monocytogenes</i> and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach |
| title_short | Understanding the Effect of Ozone on <i>Listeria monocytogenes</i> and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach |
| title_sort | understanding the effect of ozone on i listeria monocytogenes i and resident microbiota of gorgonzola cheese surface a culturomic approach |
| topic | cheese culturomics food safety <i>Listeria monocytogenes</i> MALDI-TOF MS ozone |
| url | https://www.mdpi.com/2304-8158/11/17/2640 |
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