Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic Development
Hematopoiesis in the embryo proceeds in a series of waves, with primitive erythroid-biased waves succeeded by definitive waves, within which the properties of hematopoietic stem cells (multilineage potential, self-renewal, and engraftability) gradually arise. Whereas self-renewal and engraftability...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-04-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671114000526 |
| _version_ | 1811259451742617600 |
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| author | Matthew A. Inlay Thomas Serwold Adriane Mosley John W. Fathman Ivan K. Dimov Jun Seita Irving L. Weissman |
| author_facet | Matthew A. Inlay Thomas Serwold Adriane Mosley John W. Fathman Ivan K. Dimov Jun Seita Irving L. Weissman |
| author_sort | Matthew A. Inlay |
| collection | DOAJ |
| description | Hematopoiesis in the embryo proceeds in a series of waves, with primitive erythroid-biased waves succeeded by definitive waves, within which the properties of hematopoietic stem cells (multilineage potential, self-renewal, and engraftability) gradually arise. Whereas self-renewal and engraftability have previously been examined in the embryo, multipotency has not been thoroughly addressed, especially at the single-cell level or within well-defined populations. To identify when and where clonal multilineage potential arises during embryogenesis, we developed a single-cell multipotency assay. We find that, during the initiation of definitive hematopoiesis in the embryo, a defined population of multipotent, engraftable progenitors emerges that is much more abundant within the yolk sac (YS) than the aorta-gonad-mesonephros (AGM) or fetal liver. These experiments indicate that multipotent cells appear in concert within both the YS and AGM and strongly implicate YS-derived progenitors as contributors to definitive hematopoiesis. |
| first_indexed | 2024-04-12T18:31:44Z |
| format | Article |
| id | doaj.art-168cbc8b8e9f48d6affdbf6f6b499f06 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-12T18:31:44Z |
| publishDate | 2014-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-168cbc8b8e9f48d6affdbf6f6b499f062022-12-22T03:21:04ZengElsevierStem Cell Reports2213-67112014-04-012445747210.1016/j.stemcr.2014.02.001Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic DevelopmentMatthew A. Inlay0Thomas Serwold1Adriane Mosley2John W. Fathman3Ivan K. Dimov4Jun Seita5Irving L. Weissman6Institute for Stem Cell Biology and Regenerative Medicine (ISCBRM), Stanford University, Stanford, CA 94305, USAJoslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USAInstitute for Stem Cell Biology and Regenerative Medicine (ISCBRM), Stanford University, Stanford, CA 94305, USAInstitute for Stem Cell Biology and Regenerative Medicine (ISCBRM), Stanford University, Stanford, CA 94305, USAInstitute for Stem Cell Biology and Regenerative Medicine (ISCBRM), Stanford University, Stanford, CA 94305, USAInstitute for Stem Cell Biology and Regenerative Medicine (ISCBRM), Stanford University, Stanford, CA 94305, USAInstitute for Stem Cell Biology and Regenerative Medicine (ISCBRM), Stanford University, Stanford, CA 94305, USAHematopoiesis in the embryo proceeds in a series of waves, with primitive erythroid-biased waves succeeded by definitive waves, within which the properties of hematopoietic stem cells (multilineage potential, self-renewal, and engraftability) gradually arise. Whereas self-renewal and engraftability have previously been examined in the embryo, multipotency has not been thoroughly addressed, especially at the single-cell level or within well-defined populations. To identify when and where clonal multilineage potential arises during embryogenesis, we developed a single-cell multipotency assay. We find that, during the initiation of definitive hematopoiesis in the embryo, a defined population of multipotent, engraftable progenitors emerges that is much more abundant within the yolk sac (YS) than the aorta-gonad-mesonephros (AGM) or fetal liver. These experiments indicate that multipotent cells appear in concert within both the YS and AGM and strongly implicate YS-derived progenitors as contributors to definitive hematopoiesis.http://www.sciencedirect.com/science/article/pii/S2213671114000526 |
| spellingShingle | Matthew A. Inlay Thomas Serwold Adriane Mosley John W. Fathman Ivan K. Dimov Jun Seita Irving L. Weissman Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic Development Stem Cell Reports |
| title | Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic Development |
| title_full | Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic Development |
| title_fullStr | Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic Development |
| title_full_unstemmed | Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic Development |
| title_short | Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic Development |
| title_sort | identification of multipotent progenitors that emerge prior to hematopoietic stem cells in embryonic development |
| url | http://www.sciencedirect.com/science/article/pii/S2213671114000526 |
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