A protocol for extracting immunolabeled murine cardiomyocytes of high-quality RNA by laser capture microdissection

A protocol for extracting immunolabeled murine cardiomyocytes of high-quality RNA by laser capture microdissection

Summary: We developed a highly efficient, ultrashort immunohistochemistry-laser capture microdissection (IHC-LMD) protocol, which allows microdissection of up to 250 single cardiomyocytes. Before LMD, murine hearts are excised, snap-frozen, and cryosectioned. RNA isolated from LMD material is of hig...

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Bibliographic Details
Main Authors: Abeer K. Shaalan, Georgina M. Ellison-Hughes
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Cell isolation
Microscopy
Molecular Biology
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001113

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722001113

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