Metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1-methylnaphthalene
Objectives 1-Methylnaphthalene (1-MN) is composed of 2 benzene rings and belongs to polycyclic aromatic hydrocarbons. The metabolism of 1-MN in laboratory animals and bacteria leads to the formation of 1-naphthoic acid (1-NA). Material and Methods In this study the distribution of 1-NA in lung, liv...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nofer Institute of Occupational Medicine
2022-09-01
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| Series: | International Journal of Occupational Medicine and Environmental Health |
| Subjects: | |
| Online Access: | http://ijomeh.eu/Metabolism-and-in-vitro-assessment-of-the-mutagenic-activity-of-urinary-extracts,151556,0,2.html |
| _version_ | 1797980790141747200 |
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| author | Radosław Świercz Maciej Stępnik Jolanta Gromadzińska Katarzyna Domeradzka-Gajda Joanna Roszak Wojciech Wąsowicz |
| author_facet | Radosław Świercz Maciej Stępnik Jolanta Gromadzińska Katarzyna Domeradzka-Gajda Joanna Roszak Wojciech Wąsowicz |
| author_sort | Radosław Świercz |
| collection | DOAJ |
| description | Objectives
1-Methylnaphthalene (1-MN) is composed of 2 benzene rings and belongs to polycyclic aromatic hydrocarbons. The metabolism of
1-MN in laboratory animals and bacteria leads to the formation of 1-naphthoic acid (1-NA).
Material and Methods
In this study the distribution of 1-NA in lung, liver, spleen, kidney and urinary excretion of 1-NA in rats after single and repeated inhalation exposure to 1-MN vapors were investigated.
The activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and cytochrome were measured of the rats. Genotoxic
effects were evaluated with the in vitro micronucleus test on V79 hamster fibroblasts.
Results
The concentrations of 1-NA in the tissues of rats after
single and repeated exposure to 1-MN were dependent on the exposure dose. High levels of 1-NA were found in kidneys of animals after the single
and repeated exposure to 1-MN. With an increase of 1-MN dose, an increase in the activity of cytochrome P450 (CYP1A1 and CYP1A2) was observed
in the liver of rats. Compared to control animals, significantly higher ALT activity was noted in serum of rats exposed to 1-MN. The micronuclei
frequency in V79 cells exposed to 1-MN (in the range of analyzable concentrations; i.e., 5–25 μg/ml) did not differ significantly from the vehicle
control, whereas urine extracts from rats exposed to 1-MN induced a significant increase in the frequency of micronuclei compared to urine extracts
from the group of control animals.
Conclusions
Metabolism of 1-MN in rats after the inhalation exposure leading to 1-NA was mainly observed
during the first day after the end of exposure. It is likely that 1-MN metabolites present in rat urine can induce the increased micronuclei frequency
as was shown in V79 cells. Int J Occup Med Environ Health. 2022;35(6):731–46 |
| first_indexed | 2024-04-11T05:59:21Z |
| format | Article |
| id | doaj.art-1694391d1bc242cf8dca61aa3f2faaa9 |
| institution | Directory Open Access Journal |
| issn | 1232-1087 1896-494X |
| language | English |
| last_indexed | 2024-04-11T05:59:21Z |
| publishDate | 2022-09-01 |
| publisher | Nofer Institute of Occupational Medicine |
| record_format | Article |
| series | International Journal of Occupational Medicine and Environmental Health |
| spelling | doaj.art-1694391d1bc242cf8dca61aa3f2faaa92022-12-22T04:41:47ZengNofer Institute of Occupational MedicineInternational Journal of Occupational Medicine and Environmental Health1232-10871896-494X2022-09-0135673174610.13075/ijomeh.1896.01955151556Metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1-methylnaphthaleneRadosław Świercz0https://orcid.org/0000-0002-7551-2946Maciej Stępnik1https://orcid.org/0000-0003-1586-2482Jolanta Gromadzińska2https://orcid.org/0000-0001-6878-2297Katarzyna Domeradzka-Gajda3https://orcid.org/0000-0002-4775-3793Joanna Roszak4https://orcid.org/0000-0002-0739-9443Wojciech Wąsowicz5https://orcid.org/0000-0002-2991-9040Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Translational Research)Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Translational Research)Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Biological and Environmental Monitoring)Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Translational Research)Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Translational Research)Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Biological and Environmental Monitoring)Objectives 1-Methylnaphthalene (1-MN) is composed of 2 benzene rings and belongs to polycyclic aromatic hydrocarbons. The metabolism of 1-MN in laboratory animals and bacteria leads to the formation of 1-naphthoic acid (1-NA). Material and Methods In this study the distribution of 1-NA in lung, liver, spleen, kidney and urinary excretion of 1-NA in rats after single and repeated inhalation exposure to 1-MN vapors were investigated. The activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and cytochrome were measured of the rats. Genotoxic effects were evaluated with the in vitro micronucleus test on V79 hamster fibroblasts. Results The concentrations of 1-NA in the tissues of rats after single and repeated exposure to 1-MN were dependent on the exposure dose. High levels of 1-NA were found in kidneys of animals after the single and repeated exposure to 1-MN. With an increase of 1-MN dose, an increase in the activity of cytochrome P450 (CYP1A1 and CYP1A2) was observed in the liver of rats. Compared to control animals, significantly higher ALT activity was noted in serum of rats exposed to 1-MN. The micronuclei frequency in V79 cells exposed to 1-MN (in the range of analyzable concentrations; i.e., 5–25 μg/ml) did not differ significantly from the vehicle control, whereas urine extracts from rats exposed to 1-MN induced a significant increase in the frequency of micronuclei compared to urine extracts from the group of control animals. Conclusions Metabolism of 1-MN in rats after the inhalation exposure leading to 1-NA was mainly observed during the first day after the end of exposure. It is likely that 1-MN metabolites present in rat urine can induce the increased micronuclei frequency as was shown in V79 cells. Int J Occup Med Environ Health. 2022;35(6):731–46http://ijomeh.eu/Metabolism-and-in-vitro-assessment-of-the-mutagenic-activity-of-urinary-extracts,151556,0,2.htmlratsinhalationmicronucleus testtoxicokinetics1-methylonaphthalene1-naphthoic acid |
| spellingShingle | Radosław Świercz Maciej Stępnik Jolanta Gromadzińska Katarzyna Domeradzka-Gajda Joanna Roszak Wojciech Wąsowicz Metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1-methylnaphthalene International Journal of Occupational Medicine and Environmental Health rats inhalation micronucleus test toxicokinetics 1-methylonaphthalene 1-naphthoic acid |
| title | Metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1-methylnaphthalene |
| title_full | Metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1-methylnaphthalene |
| title_fullStr | Metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1-methylnaphthalene |
| title_full_unstemmed | Metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1-methylnaphthalene |
| title_short | Metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1-methylnaphthalene |
| title_sort | metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1 methylnaphthalene |
| topic | rats inhalation micronucleus test toxicokinetics 1-methylonaphthalene 1-naphthoic acid |
| url | http://ijomeh.eu/Metabolism-and-in-vitro-assessment-of-the-mutagenic-activity-of-urinary-extracts,151556,0,2.html |
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