Facile mutant identification via a single parental backcross method and application of whole genome sequencing based mapping pipelines

Facile mutant identification via a single parental backcross method and application of whole genome sequencing based mapping pipelines

Forward genetic screens have identified numerous genes involved in development and metabolism, and remain a cornerstone of biological research. However to locate a causal mutation, the practice of crossing to a polymorphic background to generate a mapping population can be problematic if the mutant...

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Bibliographic Details
Main Authors: Robert Silas Allen, Kenlee eNakasugi, Rachel eDoran, Tony A Millar, Peter eWaterhouse
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-09-01
Series:Frontiers in Plant Science
Subjects:
Arabidopsis
mutant-mapping
NextGen-Sequencing
parental-backcross
EMS
mutant-screen.
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00362/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00362/full

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