SARS-CoV2 RT-PCR assays: In vitro comparison of 4 WHO approved protocols on clinical specimens and its implications for real laboratory practice through variant emergence

Abstract Introduction RT-PCR testing on nasopharyngeal swabs is a key component in the COVID-19 fighting, provided to use sensitive and specific SARS-CoV2 genome targets. In this study, we aimed to evaluate and to compare 4 widely used WHO approved RT-PCR protocols on real clinical specimens, to dec...

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Bibliographic Details
Main Authors: Mariem Gdoura, Imen Abouda, Mehdi Mrad, Imen Ben Dhifallah, Zeineb Belaiba, Wasfi Fares, Anissa Chouikha, Maroua Khedhiri, Kaouther Layouni, Henda Touzi, Amel Sadraoui, Walid Hammemi, Zina Meddeb, Nahed Hogga, Sihem Ben Fadhel, Sondes Haddad-Boubaker, Henda Triki
Format: Article
Language:English
Published: BMC 2022-03-01
Series:Virology Journal
Subjects:
Online Access:https://doi.org/10.1186/s12985-022-01784-4