Platycodin D restores the intestinal mechanicalbarrier by reducing endoplasmic reticulum stress-mediated apoptosis
The current study aimed to reveal the inhibitory effect of Platycodin D (PD) on intestinal cell apoptosis mediated by ER stress. Treatment with PD alleviated pathological changes of the intestine and reduced secretion of intracellular enzyme Diamine oxidase (DAO) in the upper villi of the small inte...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622004066 |
| _version_ | 1828120673166295040 |
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| author | Mei-ling Fan Kun Wei Xiao-meng Wei Jun-jie Zhang Jin-gang Hou Qiong Shen Yin-shi Sun Xin-dian Li Zi Wang Li-li Jiao Wei Li |
| author_facet | Mei-ling Fan Kun Wei Xiao-meng Wei Jun-jie Zhang Jin-gang Hou Qiong Shen Yin-shi Sun Xin-dian Li Zi Wang Li-li Jiao Wei Li |
| author_sort | Mei-ling Fan |
| collection | DOAJ |
| description | The current study aimed to reveal the inhibitory effect of Platycodin D (PD) on intestinal cell apoptosis mediated by ER stress. Treatment with PD alleviated pathological changes of the intestine and reduced secretion of intracellular enzyme Diamine oxidase (DAO) in the upper villi of the small intestinal mucosa in Cisplatin (CP) intestinal injury model and increased expression of key protein Occludin and ZO-1 of the intestinal mechanical barrier. PD inhibited ER stress in a dose-dependent in CP-caused IEC-6 cells and the level of phosphorylation of Protein kinase R-like ERK kinase (PERK) and Eukaryotic initiation factor-2 alpha (eIF2α) reduced. Besides, the ER stress inhibitor 4-PBA was used to confirm that PD alleviated the ER stress in vitro. In conclusion, PD exerted an intestinal protective effect through the inhibition of ER stress in CP-treated mice and IEC-6 cells. These data indicated that PD was a promising natural medicine for intestinal protection. |
| first_indexed | 2024-04-11T14:09:55Z |
| format | Article |
| id | doaj.art-16b82955ff374e2bbb100d2be5ee04bd |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-11T14:09:55Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-16b82955ff374e2bbb100d2be5ee04bd2022-12-22T04:19:45ZengElsevierJournal of Functional Foods1756-46462022-12-0199105336Platycodin D restores the intestinal mechanicalbarrier by reducing endoplasmic reticulum stress-mediated apoptosisMei-ling Fan0Kun Wei1Xiao-meng Wei2Jun-jie Zhang3Jin-gang Hou4Qiong Shen5Yin-shi Sun6Xin-dian Li7Zi Wang8Li-li Jiao9Wei Li10The Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130021, ChinaThe Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130021, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China; College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaKI for the Bio Century, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of KoreaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China; Corresponding authors.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; Corresponding authors.The current study aimed to reveal the inhibitory effect of Platycodin D (PD) on intestinal cell apoptosis mediated by ER stress. Treatment with PD alleviated pathological changes of the intestine and reduced secretion of intracellular enzyme Diamine oxidase (DAO) in the upper villi of the small intestinal mucosa in Cisplatin (CP) intestinal injury model and increased expression of key protein Occludin and ZO-1 of the intestinal mechanical barrier. PD inhibited ER stress in a dose-dependent in CP-caused IEC-6 cells and the level of phosphorylation of Protein kinase R-like ERK kinase (PERK) and Eukaryotic initiation factor-2 alpha (eIF2α) reduced. Besides, the ER stress inhibitor 4-PBA was used to confirm that PD alleviated the ER stress in vitro. In conclusion, PD exerted an intestinal protective effect through the inhibition of ER stress in CP-treated mice and IEC-6 cells. These data indicated that PD was a promising natural medicine for intestinal protection.http://www.sciencedirect.com/science/article/pii/S1756464622004066Platycodin DEndoplasmic reticulum stressCisplatinIntestinal mechanical barrier4-PBAIntestinal injury |
| spellingShingle | Mei-ling Fan Kun Wei Xiao-meng Wei Jun-jie Zhang Jin-gang Hou Qiong Shen Yin-shi Sun Xin-dian Li Zi Wang Li-li Jiao Wei Li Platycodin D restores the intestinal mechanicalbarrier by reducing endoplasmic reticulum stress-mediated apoptosis Journal of Functional Foods Platycodin D Endoplasmic reticulum stress Cisplatin Intestinal mechanical barrier 4-PBA Intestinal injury |
| title | Platycodin D restores the intestinal mechanicalbarrier by reducing endoplasmic reticulum stress-mediated apoptosis |
| title_full | Platycodin D restores the intestinal mechanicalbarrier by reducing endoplasmic reticulum stress-mediated apoptosis |
| title_fullStr | Platycodin D restores the intestinal mechanicalbarrier by reducing endoplasmic reticulum stress-mediated apoptosis |
| title_full_unstemmed | Platycodin D restores the intestinal mechanicalbarrier by reducing endoplasmic reticulum stress-mediated apoptosis |
| title_short | Platycodin D restores the intestinal mechanicalbarrier by reducing endoplasmic reticulum stress-mediated apoptosis |
| title_sort | platycodin d restores the intestinal mechanicalbarrier by reducing endoplasmic reticulum stress mediated apoptosis |
| topic | Platycodin D Endoplasmic reticulum stress Cisplatin Intestinal mechanical barrier 4-PBA Intestinal injury |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622004066 |
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