Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame.
α-L-Aspartyl-D-phenylalanine methyl ester (L, D-APM) and α-D-aspartyl-L-phenylalanine methyl ester (D, L-APM) are diastereomers of aspartame (N-L-α-Aspartyl-L-phenylalanine-1-methyl ester, L, L-APM). The Joint FAO/WHO Expert Committee on Food Additives has set 0.04 wt% as the maximum permitted level...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4807810?pdf=render |
| _version_ | 1818409716239302656 |
|---|---|
| author | Takashi Ohtsuki Ryoichiro Nakamura Satoru Kubo Akira Otabe Yoko Oobayashi Shoko Suzuki Mika Yoshida Mitsuya Yoshida Chiye Tatebe Kyoko Sato Hiroshi Akiyama |
| author_facet | Takashi Ohtsuki Ryoichiro Nakamura Satoru Kubo Akira Otabe Yoko Oobayashi Shoko Suzuki Mika Yoshida Mitsuya Yoshida Chiye Tatebe Kyoko Sato Hiroshi Akiyama |
| author_sort | Takashi Ohtsuki |
| collection | DOAJ |
| description | α-L-Aspartyl-D-phenylalanine methyl ester (L, D-APM) and α-D-aspartyl-L-phenylalanine methyl ester (D, L-APM) are diastereomers of aspartame (N-L-α-Aspartyl-L-phenylalanine-1-methyl ester, L, L-APM). The Joint FAO/WHO Expert Committee on Food Additives has set 0.04 wt% as the maximum permitted level of the sum of L, D-APM and D, L-APM in commercially available L, L-APM. In this study, we developed and validated a simple high-performance liquid chromatography (HPLC) method using an ODS column to determine L, D-APM and D, L-APM in L, L-APM. The limits of detection and quantification, respectively, of L, D-APM and D, L-APM were found to be 0.0012 wt% and 0.004 wt%. This method gave excellent accuracy, repeatability, and reproducibility in a recovery test performed on five different days. Moreover, the method was successfully applied to the determination of these diastereomers in commercial L, L-APM samples. Thus, the developed method is a simple, useful, and practical tool for determining L, D-APM and D, L-APM levels in L, L-APM. |
| first_indexed | 2024-12-14T10:04:03Z |
| format | Article |
| id | doaj.art-16b8f0b0b40a48ccbf3adccce1c1bee4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T10:04:03Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-16b8f0b0b40a48ccbf3adccce1c1bee42022-12-21T23:07:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015217410.1371/journal.pone.0152174Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame.Takashi OhtsukiRyoichiro NakamuraSatoru KuboAkira OtabeYoko OobayashiShoko SuzukiMika YoshidaMitsuya YoshidaChiye TatebeKyoko SatoHiroshi Akiyamaα-L-Aspartyl-D-phenylalanine methyl ester (L, D-APM) and α-D-aspartyl-L-phenylalanine methyl ester (D, L-APM) are diastereomers of aspartame (N-L-α-Aspartyl-L-phenylalanine-1-methyl ester, L, L-APM). The Joint FAO/WHO Expert Committee on Food Additives has set 0.04 wt% as the maximum permitted level of the sum of L, D-APM and D, L-APM in commercially available L, L-APM. In this study, we developed and validated a simple high-performance liquid chromatography (HPLC) method using an ODS column to determine L, D-APM and D, L-APM in L, L-APM. The limits of detection and quantification, respectively, of L, D-APM and D, L-APM were found to be 0.0012 wt% and 0.004 wt%. This method gave excellent accuracy, repeatability, and reproducibility in a recovery test performed on five different days. Moreover, the method was successfully applied to the determination of these diastereomers in commercial L, L-APM samples. Thus, the developed method is a simple, useful, and practical tool for determining L, D-APM and D, L-APM levels in L, L-APM.http://europepmc.org/articles/PMC4807810?pdf=render |
| spellingShingle | Takashi Ohtsuki Ryoichiro Nakamura Satoru Kubo Akira Otabe Yoko Oobayashi Shoko Suzuki Mika Yoshida Mitsuya Yoshida Chiye Tatebe Kyoko Sato Hiroshi Akiyama Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame. PLoS ONE |
| title | Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame. |
| title_full | Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame. |
| title_fullStr | Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame. |
| title_full_unstemmed | Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame. |
| title_short | Development of an HPLC Method with an ODS Column to Determine Low Levels of Aspartame Diastereomers in Aspartame. |
| title_sort | development of an hplc method with an ods column to determine low levels of aspartame diastereomers in aspartame |
| url | http://europepmc.org/articles/PMC4807810?pdf=render |
| work_keys_str_mv | AT takashiohtsuki developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT ryoichironakamura developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT satorukubo developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT akiraotabe developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT yokooobayashi developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT shokosuzuki developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT mikayoshida developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT mitsuyayoshida developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT chiyetatebe developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT kyokosato developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame AT hiroshiakiyama developmentofanhplcmethodwithanodscolumntodeterminelowlevelsofaspartamediastereomersinaspartame |