Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass Spectrometry
Consumption of boiled crayfish may lead to Haff disease (HD), which is considered to result from an unidentified toxin, although the etiology is still obscure. Profiling of the N-glycome in HD would assist in deciphering the underlying molecular mechanism of the disease, whereas HD-associated glycos...
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Elsevier
2023-07-01
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| Series: | Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095809922007597 |
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| author | Si Liu Yuanyuan Liu Jiajing Lin Bi-Feng Liu Zhenyu He Xiaomin Wu Xin Liu |
| author_facet | Si Liu Yuanyuan Liu Jiajing Lin Bi-Feng Liu Zhenyu He Xiaomin Wu Xin Liu |
| author_sort | Si Liu |
| collection | DOAJ |
| description | Consumption of boiled crayfish may lead to Haff disease (HD), which is considered to result from an unidentified toxin, although the etiology is still obscure. Profiling of the N-glycome in HD would assist in deciphering the underlying molecular mechanism of the disease, whereas HD-associated glycosylation has never been explored. Herein, we enrolled 90 serum samples with HD patients and healthy controls from the Wuhan Center for Disease Control & Prevention between 2019 and 2020. N-glycome profiles of both serum and serum-derived immunoglobulin G (IgG) in HD were characterized by means of high-throughput-based orthogonal mass spectrometry. It was observed that HD is associated with an increase in the core fucosylation and mono-galactosylation of total serum glycoproteins. The serum level of IgG was found to serve as a good indicator for HD patients. In addition, differential galactosylation and sialylation of IgG were strongly correlated with HD. It was notable that the changes in the galactosylation and sialylation of IgG1 and IgG2 were subclass specific. Interestingly, altered sialylation and galactosylation of IgG2 or IgG3/4 strongly correlated with clinical markers for HD. Our study reveals the association of differential IgG N-glycosylation with HD, providing new insight into the etiology of this rare disease. |
| first_indexed | 2024-03-11T21:34:30Z |
| format | Article |
| id | doaj.art-16e3029e85684cd9afd347d91293cae2 |
| institution | Directory Open Access Journal |
| issn | 2095-8099 |
| language | English |
| last_indexed | 2024-03-11T21:34:30Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
| series | Engineering |
| spelling | doaj.art-16e3029e85684cd9afd347d91293cae22023-09-27T04:42:44ZengElsevierEngineering2095-80992023-07-01266373Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass SpectrometrySi Liu0Yuanyuan Liu1Jiajing Lin2Bi-Feng Liu3Zhenyu He4Xiaomin Wu5Xin Liu6The Key Laboratory for Biomedical Photonics of the MOE at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics and Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaThe Key Laboratory for Biomedical Photonics of the MOE at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics and Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaThe Key Laboratory for Biomedical Photonics of the MOE at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics and Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaThe Key Laboratory for Biomedical Photonics of the MOE at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics and Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaWuhan Center for Disease Control & Prevention, Wuhan 430015, ChinaWuhan Center for Disease Control & Prevention, Wuhan 430015, China; Corresponding authors.The Key Laboratory for Biomedical Photonics of the MOE at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics and Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; Corresponding authors.Consumption of boiled crayfish may lead to Haff disease (HD), which is considered to result from an unidentified toxin, although the etiology is still obscure. Profiling of the N-glycome in HD would assist in deciphering the underlying molecular mechanism of the disease, whereas HD-associated glycosylation has never been explored. Herein, we enrolled 90 serum samples with HD patients and healthy controls from the Wuhan Center for Disease Control & Prevention between 2019 and 2020. N-glycome profiles of both serum and serum-derived immunoglobulin G (IgG) in HD were characterized by means of high-throughput-based orthogonal mass spectrometry. It was observed that HD is associated with an increase in the core fucosylation and mono-galactosylation of total serum glycoproteins. The serum level of IgG was found to serve as a good indicator for HD patients. In addition, differential galactosylation and sialylation of IgG were strongly correlated with HD. It was notable that the changes in the galactosylation and sialylation of IgG1 and IgG2 were subclass specific. Interestingly, altered sialylation and galactosylation of IgG2 or IgG3/4 strongly correlated with clinical markers for HD. Our study reveals the association of differential IgG N-glycosylation with HD, providing new insight into the etiology of this rare disease.http://www.sciencedirect.com/science/article/pii/S2095809922007597Haff diseaseSerumImmunoglobulin GGlycosylationDisease pathogenesis |
| spellingShingle | Si Liu Yuanyuan Liu Jiajing Lin Bi-Feng Liu Zhenyu He Xiaomin Wu Xin Liu Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass Spectrometry Engineering Haff disease Serum Immunoglobulin G Glycosylation Disease pathogenesis |
| title | Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass Spectrometry |
| title_full | Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass Spectrometry |
| title_fullStr | Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass Spectrometry |
| title_full_unstemmed | Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass Spectrometry |
| title_short | Novel Insight into the Etiology of Haff Disease by Mapping the N-Glycome with Orthogonal Mass Spectrometry |
| title_sort | novel insight into the etiology of haff disease by mapping the n glycome with orthogonal mass spectrometry |
| topic | Haff disease Serum Immunoglobulin G Glycosylation Disease pathogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2095809922007597 |
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