LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4
Abstract Background The present study aimed to verify whether long noncoding RNA (lncRNA) MALAT1 is involved in brain tissue damage induced by ischemia-reperfusion injury, and to explore the mechanism by which MALAT1 regulates aquaporin 4 (AQP4). Methods In this study, we established glucose depriva...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Journal of Biomedical Science |
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| Online Access: | http://link.springer.com/article/10.1186/s12929-020-00635-0 |
| _version_ | 1818594420410286080 |
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| author | Hongwei Wang Xiaoxiao Zheng Jing Jin Li Zheng Ting Guan Yangfan Huo Shufen Xie Ying Wu Wei Chen |
| author_facet | Hongwei Wang Xiaoxiao Zheng Jing Jin Li Zheng Ting Guan Yangfan Huo Shufen Xie Ying Wu Wei Chen |
| author_sort | Hongwei Wang |
| collection | DOAJ |
| description | Abstract Background The present study aimed to verify whether long noncoding RNA (lncRNA) MALAT1 is involved in brain tissue damage induced by ischemia-reperfusion injury, and to explore the mechanism by which MALAT1 regulates aquaporin 4 (AQP4). Methods In this study, we established glucose deprivation (OGD)/reoxygenation (RX) astrocyte cell model and middle cerebral artery occlusion (MCAO)/reperfusion mouse model in vitro and in vivo. Then cell counting kit-8 assay, flow cytometry analysis, Triphenyltetrazolium chloride (TTC) staining, and western blotting were used to determine cell viability, cell apoptosis, cerebral infarction volume, and the abundance of AQP4, respectively. Results We found that the level of MALAT1 was significantly upregulated in both the MCAO/reperfusion model and OGD/RX model. Knockdown of MALAT1 increased cell viability and reduced cell apoptosis in MA-C cells, while an AQP4 siRNA combined with a siRNA targeting MALAT1 could not enhance this effect. Further experiments showed that MALAT1 positively regulated AQP4 expression via miR-145. The MALAT1 siRNA did not alleviate the exacerbation of damage after miR-145 inhibitor action. However, an miR-145 inhibitor reversed the protection effects of MALAT1, indicating that MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145. TTC staining showed that the infracted area of whole brain was significantly attenuated in treated with sh-MALAT1 group in vivo. Conclusion Taken together, our study confirmed that MALAT1 promotes cerebral ischemia-reperfusion injury by affecting AQP4 expression through competitively binding miR-145, indicating that MALAT1 might be a new therapeutic target for treatment cerebral ischemic stroke. |
| first_indexed | 2024-12-16T10:59:50Z |
| format | Article |
| id | doaj.art-16eb277f54cd4b388db27eb06452e457 |
| institution | Directory Open Access Journal |
| issn | 1423-0127 |
| language | English |
| last_indexed | 2024-12-16T10:59:50Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biomedical Science |
| spelling | doaj.art-16eb277f54cd4b388db27eb06452e4572022-12-21T22:34:01ZengBMCJournal of Biomedical Science1423-01272020-03-0127111210.1186/s12929-020-00635-0LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4Hongwei Wang0Xiaoxiao Zheng1Jing Jin2Li Zheng3Ting Guan4Yangfan Huo5Shufen Xie6Ying Wu7Wei Chen8Department of anesthesiology, Tongde hospital of Zhejiang ProvinceCancer Institute of Integrated traditional Chinese and Western Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tongde hospital of Zhejiang ProvinceDepartment of Brain Surgery,The First Affiliated Hospital, Zhejiang University School of MedicineCancer Institute of Integrated traditional Chinese and Western Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tongde hospital of Zhejiang ProvinceDepartment of anesthesiology, Tongde hospital of Zhejiang ProvinceDepartment of anesthesiology, Tongde hospital of Zhejiang ProvinceDepartment of anesthesiology, Tongde hospital of Zhejiang ProvinceCancer Institute of Integrated traditional Chinese and Western Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tongde hospital of Zhejiang ProvinceCancer Institute of Integrated traditional Chinese and Western Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tongde hospital of Zhejiang ProvinceAbstract Background The present study aimed to verify whether long noncoding RNA (lncRNA) MALAT1 is involved in brain tissue damage induced by ischemia-reperfusion injury, and to explore the mechanism by which MALAT1 regulates aquaporin 4 (AQP4). Methods In this study, we established glucose deprivation (OGD)/reoxygenation (RX) astrocyte cell model and middle cerebral artery occlusion (MCAO)/reperfusion mouse model in vitro and in vivo. Then cell counting kit-8 assay, flow cytometry analysis, Triphenyltetrazolium chloride (TTC) staining, and western blotting were used to determine cell viability, cell apoptosis, cerebral infarction volume, and the abundance of AQP4, respectively. Results We found that the level of MALAT1 was significantly upregulated in both the MCAO/reperfusion model and OGD/RX model. Knockdown of MALAT1 increased cell viability and reduced cell apoptosis in MA-C cells, while an AQP4 siRNA combined with a siRNA targeting MALAT1 could not enhance this effect. Further experiments showed that MALAT1 positively regulated AQP4 expression via miR-145. The MALAT1 siRNA did not alleviate the exacerbation of damage after miR-145 inhibitor action. However, an miR-145 inhibitor reversed the protection effects of MALAT1, indicating that MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145. TTC staining showed that the infracted area of whole brain was significantly attenuated in treated with sh-MALAT1 group in vivo. Conclusion Taken together, our study confirmed that MALAT1 promotes cerebral ischemia-reperfusion injury by affecting AQP4 expression through competitively binding miR-145, indicating that MALAT1 might be a new therapeutic target for treatment cerebral ischemic stroke.http://link.springer.com/article/10.1186/s12929-020-00635-0MALAT1miR-145Cerebral ischemia-reperfusion injuryAQP4 |
| spellingShingle | Hongwei Wang Xiaoxiao Zheng Jing Jin Li Zheng Ting Guan Yangfan Huo Shufen Xie Ying Wu Wei Chen LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4 Journal of Biomedical Science MALAT1 miR-145 Cerebral ischemia-reperfusion injury AQP4 |
| title | LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4 |
| title_full | LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4 |
| title_fullStr | LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4 |
| title_full_unstemmed | LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4 |
| title_short | LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4 |
| title_sort | lncrna malat1 silencing protects against cerebral ischemia reperfusion injury through mir 145 to regulate aqp4 |
| topic | MALAT1 miR-145 Cerebral ischemia-reperfusion injury AQP4 |
| url | http://link.springer.com/article/10.1186/s12929-020-00635-0 |
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