Protocol for generating mutant zebrafish using CRISPR-Cas9 followed by quantitative evaluation of vascular formation
Summary: The use of vascular-specific transgenic zebrafish provides advantages for identifying new mutations affecting angiogenesis and vascular development. Here, we present a protocol for establishing, screening, and phenotyping CRISPR-Cas9-based mutagenesis in fluorescently labeled transgenic zeb...
| Huvudupphovsmän: | , , , |
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| Materialtyp: | Artikel |
| Språk: | English |
| Publicerad: |
Elsevier
2023-12-01
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| Serie: | STAR Protocols |
| Ämnen: | |
| Länkar: | http://www.sciencedirect.com/science/article/pii/S2666166723007207 |