Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study
The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic f...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-02-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/10/2/85 |
| _version_ | 1811301229066715136 |
|---|---|
| author | Juan Carlos Quintana-Castillo Leidy Johana Vargas Cesar Segura Sebastián Estrada-Gómez Julio César Bueno-Sánchez Juan Carlos Alarcón |
| author_facet | Juan Carlos Quintana-Castillo Leidy Johana Vargas Cesar Segura Sebastián Estrada-Gómez Julio César Bueno-Sánchez Juan Carlos Alarcón |
| author_sort | Juan Carlos Quintana-Castillo |
| collection | DOAJ |
| description | The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. Identification and analysis of C. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which the crotoxin complex was the most abundant (64.71%). Immunorecognition tests of C. d. cumanensis showed that the use of a commercial antivenom manufactured in Mexico resulted in immunoreactivity. |
| first_indexed | 2024-04-13T07:05:08Z |
| format | Article |
| id | doaj.art-16fb79873a734af4b8e6c5c63f8dd25e |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-13T07:05:08Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-16fb79873a734af4b8e6c5c63f8dd25e2022-12-22T02:57:00ZengMDPI AGToxins2072-66512018-02-011028510.3390/toxins10020085toxins10020085Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic StudyJuan Carlos Quintana-Castillo0Leidy Johana Vargas1Cesar Segura2Sebastián Estrada-Gómez3Julio César Bueno-Sánchez4Juan Carlos Alarcón5School of Medicine, Universidad Cooperativa de Colombia, Sede Medellín, Street 50 A N° 41-20, Medellín 050010, ColombiaSchool of Medicine, Universidad Cooperativa de Colombia, Sede Medellín, Street 50 A N° 41-20, Medellín 050010, ColombiaMalaria Group, School of Medicine, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, ColombiaOphidism/Scorpionism Program, Food and Pharmaceutical Sciences Faculty, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, ColombiaReproduction Group, School of Medicine, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, ColombiaOphidism/Scorpionism Program, Food and Pharmaceutical Sciences Faculty, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, ColombiaThe Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. Identification and analysis of C. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which the crotoxin complex was the most abundant (64.71%). Immunorecognition tests of C. d. cumanensis showed that the use of a commercial antivenom manufactured in Mexico resulted in immunoreactivity.http://www.mdpi.com/2072-6651/10/2/85C. d. cumanensis venomantivenomHPLC-nESI-MS/MSpeptide identificationKey ContributionThis work carried out a proteomic analysis of C. d. cumanensis from Colombia by using HPLC-nESI MS/MS and subsequently was evaluated the effect of a commercial antivenom against the venom |
| spellingShingle | Juan Carlos Quintana-Castillo Leidy Johana Vargas Cesar Segura Sebastián Estrada-Gómez Julio César Bueno-Sánchez Juan Carlos Alarcón Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study Toxins C. d. cumanensis venom antivenom HPLC-nESI-MS/MS peptide identification Key Contribution This work carried out a proteomic analysis of C. d. cumanensis from Colombia by using HPLC-nESI MS/MS and subsequently was evaluated the effect of a commercial antivenom against the venom |
| title | Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title_full | Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title_fullStr | Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title_full_unstemmed | Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title_short | Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study |
| title_sort | characterization of the venom of c d cumanesis of colombia proteomic analysis and antivenomic study |
| topic | C. d. cumanensis venom antivenom HPLC-nESI-MS/MS peptide identification Key Contribution This work carried out a proteomic analysis of C. d. cumanensis from Colombia by using HPLC-nESI MS/MS and subsequently was evaluated the effect of a commercial antivenom against the venom |
| url | http://www.mdpi.com/2072-6651/10/2/85 |
| work_keys_str_mv | AT juancarlosquintanacastillo characterizationofthevenomofcdcumanesisofcolombiaproteomicanalysisandantivenomicstudy AT leidyjohanavargas characterizationofthevenomofcdcumanesisofcolombiaproteomicanalysisandantivenomicstudy AT cesarsegura characterizationofthevenomofcdcumanesisofcolombiaproteomicanalysisandantivenomicstudy AT sebastianestradagomez characterizationofthevenomofcdcumanesisofcolombiaproteomicanalysisandantivenomicstudy AT juliocesarbuenosanchez characterizationofthevenomofcdcumanesisofcolombiaproteomicanalysisandantivenomicstudy AT juancarlosalarcon characterizationofthevenomofcdcumanesisofcolombiaproteomicanalysisandantivenomicstudy |