Development of a Spectrophotometric Method for Monitoring Angiotensin-Converting Enzyme in Dairy Products
The angiotensin-converting enzyme (ACE) regulates the levels of blood pressure through generation of angiotensin-II from angiotensin-I. It is of great importance to have a reliable and yet simple method for a quantitative determination ACE inhibitory peptides in whey of milk products. A rapid, simpl...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2013-01-01
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| Series: | Pakistan Veterinary Journal |
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| Online Access: | http://pvj.com.pk/pdf-files/33_1/14-18.pdf |
| _version_ | 1818482078080040960 |
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| author | Julijana Tomovska*, S. Presilski, N. Gjorgievski, N. Tomovska1, M. S. Qureshi2 and N. P. Bozinovska3 |
| author_facet | Julijana Tomovska*, S. Presilski, N. Gjorgievski, N. Tomovska1, M. S. Qureshi2 and N. P. Bozinovska3 |
| author_sort | Julijana Tomovska*, S. Presilski, N. Gjorgievski, N. Tomovska1, M. S. Qureshi2 and N. P. Bozinovska3 |
| collection | DOAJ |
| description | The angiotensin-converting enzyme (ACE) regulates the levels of blood pressure through generation of angiotensin-II from angiotensin-I. It is of great importance to have a reliable and yet simple method for a quantitative determination ACE inhibitory peptides in whey of milk products. A rapid, simple, sensitive and accurate spectrophotometric kinetic method has been developed for determination of ACE inhibitory peptides, using competitive inhibition. Samples of dairy product from the market were used for the determination of ACE inhibitory peptides in whey. Holmquist’s kinetic method was used for determining ACE inhibitory activity in blood serum and Ronca-Testoni method was used for the determination of ACE inhibitory activity in whey. Enzymatic inhibition activity was determined using 0.8 mmol/L FAPGG (N-[3-(Furyl) –Acryloyl]-L-Phenylalanyl Glycyl Glycyne) as the substrate in 50 mmol/L Tris buffer at pH 8.2 at 37°C and a standard serum containing ACE. First, a solution of whey was mixed in a 1 to 10 ratio with serum (elevation) containing high ACE activity. The enzymatic activity was determined by monitoring the decrease in absorbance at 340 nm as result of hydrolysis of the substrate. The concentration of ACE inhibitory peptides was determined from a standard curve of inhibitor concentration versus percent of ACE inhibition. The study suggests that the method possesses good reproducibility and accuracy. The linear range enabled determination of high enzymatic activity of ACE and all ACE inhibitory peptides from dairy products act as competitive inhibitors. |
| first_indexed | 2024-12-10T11:43:24Z |
| format | Article |
| id | doaj.art-17140e69e89a4ac7ba8d3339b2d48c6d |
| institution | Directory Open Access Journal |
| issn | 0253-8318 |
| language | English |
| last_indexed | 2024-12-10T11:43:24Z |
| publishDate | 2013-01-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-17140e69e89a4ac7ba8d3339b2d48c6d2022-12-22T01:50:10ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182013-01-013311418Development of a Spectrophotometric Method for Monitoring Angiotensin-Converting Enzyme in Dairy ProductsJulijana Tomovska*, S. Presilski, N. Gjorgievski, N. Tomovska1, M. S. Qureshi2 and N. P. Bozinovska3The angiotensin-converting enzyme (ACE) regulates the levels of blood pressure through generation of angiotensin-II from angiotensin-I. It is of great importance to have a reliable and yet simple method for a quantitative determination ACE inhibitory peptides in whey of milk products. A rapid, simple, sensitive and accurate spectrophotometric kinetic method has been developed for determination of ACE inhibitory peptides, using competitive inhibition. Samples of dairy product from the market were used for the determination of ACE inhibitory peptides in whey. Holmquist’s kinetic method was used for determining ACE inhibitory activity in blood serum and Ronca-Testoni method was used for the determination of ACE inhibitory activity in whey. Enzymatic inhibition activity was determined using 0.8 mmol/L FAPGG (N-[3-(Furyl) –Acryloyl]-L-Phenylalanyl Glycyl Glycyne) as the substrate in 50 mmol/L Tris buffer at pH 8.2 at 37°C and a standard serum containing ACE. First, a solution of whey was mixed in a 1 to 10 ratio with serum (elevation) containing high ACE activity. The enzymatic activity was determined by monitoring the decrease in absorbance at 340 nm as result of hydrolysis of the substrate. The concentration of ACE inhibitory peptides was determined from a standard curve of inhibitor concentration versus percent of ACE inhibition. The study suggests that the method possesses good reproducibility and accuracy. The linear range enabled determination of high enzymatic activity of ACE and all ACE inhibitory peptides from dairy products act as competitive inhibitors.http://pvj.com.pk/pdf-files/33_1/14-18.pdfAngiotensin-convertingCompetitive inhibitorsDairy productsEnzymeEnzymatic activitySpectrophotometry |
| spellingShingle | Julijana Tomovska*, S. Presilski, N. Gjorgievski, N. Tomovska1, M. S. Qureshi2 and N. P. Bozinovska3 Development of a Spectrophotometric Method for Monitoring Angiotensin-Converting Enzyme in Dairy Products Pakistan Veterinary Journal Angiotensin-converting Competitive inhibitors Dairy products Enzyme Enzymatic activity Spectrophotometry |
| title | Development of a Spectrophotometric Method for Monitoring Angiotensin-Converting Enzyme in Dairy Products |
| title_full | Development of a Spectrophotometric Method for Monitoring Angiotensin-Converting Enzyme in Dairy Products |
| title_fullStr | Development of a Spectrophotometric Method for Monitoring Angiotensin-Converting Enzyme in Dairy Products |
| title_full_unstemmed | Development of a Spectrophotometric Method for Monitoring Angiotensin-Converting Enzyme in Dairy Products |
| title_short | Development of a Spectrophotometric Method for Monitoring Angiotensin-Converting Enzyme in Dairy Products |
| title_sort | development of a spectrophotometric method for monitoring angiotensin converting enzyme in dairy products |
| topic | Angiotensin-converting Competitive inhibitors Dairy products Enzyme Enzymatic activity Spectrophotometry |
| url | http://pvj.com.pk/pdf-files/33_1/14-18.pdf |
| work_keys_str_mv | AT julijanatomovskaspresilskingjorgievskintomovska1msqureshi2andnpbozinovska3 developmentofaspectrophotometricmethodformonitoringangiotensinconvertingenzymeindairyproducts |