Gamma-irradiated influenza A virus provides adjuvant activity to a co-administered poorly immunogenic SFV vaccine in mice.
Many currently available inactivated vaccines require 'adjuvants' to maximise the protective immune responses generated against the antigens of interest. Recent studies in mice with gamma-irradiated influenza A virus (γ-FLU) have shown its superior efficacy compared to other forms of inact...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-06-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00267/full |
| _version_ | 1818494300633169920 |
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| author | Rachelle eBabb Jennifer eChan Jasmine E Khairat Yoichi eFuruya Mohammed eAlsharifi |
| author_facet | Rachelle eBabb Jennifer eChan Jasmine E Khairat Yoichi eFuruya Mohammed eAlsharifi |
| author_sort | Rachelle eBabb |
| collection | DOAJ |
| description | Many currently available inactivated vaccines require 'adjuvants' to maximise the protective immune responses generated against the antigens of interest. Recent studies in mice with gamma-irradiated influenza A virus (γ-FLU) have shown its superior efficacy compared to other forms of inactivated FLU vaccines and its ability to induce both potent type-I interferon (IFN-I) responses and the IFN-I associated partial lymphocyte activation. Commonly, IFN-I responses induced by adjuvants, combined in vaccine preparations, have been shown to effectively enhance the immunogenicity of the antigens of interest. Therefore, we investigated the potential adjuvant activity of γ-FLU and the possible effect on antibody responses against co-administrated antigens, using gamma-irradiated Semliki Forest Virus (γ-SFV) as the experimental vaccine in mice. Our data show that co-vaccination with γ-FLU and γ-SFV resulted in enhanced SFV-specific antibody responses in terms of increased titres by 6 fold and greater neutralisation efficacy, when compared to vaccination with γ-SFV alone. This study provides promising evidence related to the possible use of γ-FLU as an adjuvant to poorly immunogenic vaccines without compromising the vaccine efficacy of γ-FLU. |
| first_indexed | 2024-12-10T18:04:46Z |
| format | Article |
| id | doaj.art-173fba0ee13b4d90ad45baf879ae88c9 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-10T18:04:46Z |
| publishDate | 2014-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-173fba0ee13b4d90ad45baf879ae88c92022-12-22T01:38:38ZengFrontiers Media S.A.Frontiers in Immunology1664-32242014-06-01510.3389/fimmu.2014.0026791942Gamma-irradiated influenza A virus provides adjuvant activity to a co-administered poorly immunogenic SFV vaccine in mice.Rachelle eBabb0Jennifer eChan1Jasmine E Khairat2Yoichi eFuruya3Mohammed eAlsharifi4Centre for Molecular Pathology, The University of AdelaideCentre for Molecular Pathology, The University of AdelaideCentre for Molecular Pathology, The University of AdelaideThe John Curtin School of Medical Research, Australian National UniversityCentre for Molecular Pathology, The University of AdelaideMany currently available inactivated vaccines require 'adjuvants' to maximise the protective immune responses generated against the antigens of interest. Recent studies in mice with gamma-irradiated influenza A virus (γ-FLU) have shown its superior efficacy compared to other forms of inactivated FLU vaccines and its ability to induce both potent type-I interferon (IFN-I) responses and the IFN-I associated partial lymphocyte activation. Commonly, IFN-I responses induced by adjuvants, combined in vaccine preparations, have been shown to effectively enhance the immunogenicity of the antigens of interest. Therefore, we investigated the potential adjuvant activity of γ-FLU and the possible effect on antibody responses against co-administrated antigens, using gamma-irradiated Semliki Forest Virus (γ-SFV) as the experimental vaccine in mice. Our data show that co-vaccination with γ-FLU and γ-SFV resulted in enhanced SFV-specific antibody responses in terms of increased titres by 6 fold and greater neutralisation efficacy, when compared to vaccination with γ-SFV alone. This study provides promising evidence related to the possible use of γ-FLU as an adjuvant to poorly immunogenic vaccines without compromising the vaccine efficacy of γ-FLU.http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00267/fullAntibodiesInfluenza Vaccinesadjuvantscombined vaccinegamma-irradiation |
| spellingShingle | Rachelle eBabb Jennifer eChan Jasmine E Khairat Yoichi eFuruya Mohammed eAlsharifi Gamma-irradiated influenza A virus provides adjuvant activity to a co-administered poorly immunogenic SFV vaccine in mice. Frontiers in Immunology Antibodies Influenza Vaccines adjuvants combined vaccine gamma-irradiation |
| title | Gamma-irradiated influenza A virus provides adjuvant activity to a co-administered poorly immunogenic SFV vaccine in mice. |
| title_full | Gamma-irradiated influenza A virus provides adjuvant activity to a co-administered poorly immunogenic SFV vaccine in mice. |
| title_fullStr | Gamma-irradiated influenza A virus provides adjuvant activity to a co-administered poorly immunogenic SFV vaccine in mice. |
| title_full_unstemmed | Gamma-irradiated influenza A virus provides adjuvant activity to a co-administered poorly immunogenic SFV vaccine in mice. |
| title_short | Gamma-irradiated influenza A virus provides adjuvant activity to a co-administered poorly immunogenic SFV vaccine in mice. |
| title_sort | gamma irradiated influenza a virus provides adjuvant activity to a co administered poorly immunogenic sfv vaccine in mice |
| topic | Antibodies Influenza Vaccines adjuvants combined vaccine gamma-irradiation |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00267/full |
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