Expression and Purification of Recombinant GHK Tripeptides Are Able to Protect against Acute Cardiotoxicity from Exposure to Waterborne-Copper in Zebrafish

Expression and Purification of Recombinant GHK Tripeptides Are Able to Protect against Acute Cardiotoxicity from Exposure to Waterborne-Copper in Zebrafish

In this study, an alternative method is developed to replace chemical synthesis to produce glycyl-histidyl-lysine (GHK) tripeptides with a bacterial fermentation system. The target GHK tripeptides are cloned into expression plasmids carrying histidine-glutathione-S-transferase (GST) double tags and...

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Bibliographic Details
Main Authors: Chung-Der Hsiao, Hsin-Hui Wu, Nemi Malhotra, Yen-Ching Liu, Ying-Hsuan Wu, Yu-Nung Lin, Ferry Saputra, Fiorency Santoso, Kelvin H.-C. Chen
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Biomolecules
Subjects:
zebrafish
GHK tripeptide
cardiotoxicity
copper
protein expression
Online Access:https://www.mdpi.com/2218-273X/10/9/1202

Internet

https://www.mdpi.com/2218-273X/10/9/1202

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