Benchmarking glycoform-resolved affinity separation – mass spectrometry assays for studying FcγRIIIa binding

Benchmarking glycoform-resolved affinity separation – mass spectrometry assays for studying FcγRIIIa binding

The antibody- FcγRIIIa interaction triggers key immunological responses such as antibody dependent cellular cytotoxicity (ADCC), making it highly important for therapeutic mAbs. Due to the direct glycan-glycan interaction with FcγRIIIa receptor, differences in antibody glycosylation can drastically...

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Bibliographic Details
Main Authors: Christoph Gstöttner, Steffen Lippold, Michaela Hook, Feng Yang, Markus Haberger, Manfred Wuhrer, David Falck, Tilman Schlothauer, Elena Domínguez-Vega
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-02-01
Series:Frontiers in Immunology
Subjects:
affinity capillary electrophoresis
affinity chromatography
mass spectrometry
FcγRIIIA receptor
monoclonal antibody
glycosylation
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2024.1347871/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2024.1347871/full

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