CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery
The short release half-life of carbon monoxide (CO) is a major obstacle to the effective therapeutic use of carbon monoxide-releasing molecule-2 (CORM-2). The potential of CORM-2-entrapped ultradeformable liposomes (CORM-2-UDLs) to enhance the release half-life of CO and alleviate skin inflammation...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383520305931 |
| _version_ | 1818591621132845056 |
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| author | Gwan-Yeong Lee Alam Zeb Eun-Hye Kim Beomseon Suh Young-Jun Shin Donghyun Kim Kyoung-Won Kim Yeong-Hwan Choe Ho-Ik Choi Cheol-Ho Lee Omer Salman Qureshi In-Bo Han Sun-Young Chang Ok-Nam Bae Jin-Ki Kim |
| author_facet | Gwan-Yeong Lee Alam Zeb Eun-Hye Kim Beomseon Suh Young-Jun Shin Donghyun Kim Kyoung-Won Kim Yeong-Hwan Choe Ho-Ik Choi Cheol-Ho Lee Omer Salman Qureshi In-Bo Han Sun-Young Chang Ok-Nam Bae Jin-Ki Kim |
| author_sort | Gwan-Yeong Lee |
| collection | DOAJ |
| description | The short release half-life of carbon monoxide (CO) is a major obstacle to the effective therapeutic use of carbon monoxide-releasing molecule-2 (CORM-2). The potential of CORM-2-entrapped ultradeformable liposomes (CORM-2-UDLs) to enhance the release half-life of CO and alleviate skin inflammation was investigated in the present study. CORM-2-UDLs were prepared by using soy phosphatidylcholine to form lipid bilayers and Tween 80 as an edge activator. The deformability of CORM-2-UDLs was measured and compared with that of conventional liposomes by passing formulations through a filter device at a constant pressure. The release profile of CO from CORM-2-UDLs was evaluated by myoglobin assay. In vitro and in vivo anti-inflammatory effects of CORM-2-UDLs were assessed in lipopolysaccharide-stimulated macrophages and TPA-induced ear edema model, respectively. The deformability of the optimized CORM-2-UDLs was 2.3 times higher than conventional liposomes. CORM-2-UDLs significantly prolonged the release half-life of CO from 30 s in a CORM-2 solution to 21.6 min. CORM-2-UDLs demonstrated in vitro anti-inflammatory activity by decreasing nitrite production and pro-inflammatory cytokine levels. Furthermore, CORM-2-UDLs successfully ameliorated skin inflammation by reducing ear edema, pathological scores, neutrophil accumulation, and inflammatory cytokines expression. The results demonstrate that CORM-2-UDLs could be used as promising therapeutics against acute skin inflammation. |
| first_indexed | 2024-12-16T10:15:21Z |
| format | Article |
| id | doaj.art-17892d56a06e492384130a87cc395e98 |
| institution | Directory Open Access Journal |
| issn | 2211-3835 |
| language | English |
| last_indexed | 2024-12-16T10:15:21Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-17892d56a06e492384130a87cc395e982022-12-21T22:35:28ZengElsevierActa Pharmaceutica Sinica B2211-38352020-12-01101223622373CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO deliveryGwan-Yeong Lee0Alam Zeb1Eun-Hye Kim2Beomseon Suh3Young-Jun Shin4Donghyun Kim5Kyoung-Won Kim6Yeong-Hwan Choe7Ho-Ik Choi8Cheol-Ho Lee9Omer Salman Qureshi10In-Bo Han11Sun-Young Chang12Ok-Nam Bae13Jin-Ki Kim14College of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of Korea; Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad 44000, PakistanCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of KoreaDepartment of Pharmacy, Forman Christian College, Lahore 54600, PakistanDepartment of Neurosurgery, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam 13496, Republic of KoreaCollege of Pharmacy, Ajou University, Suwon 16499, Republic of KoreaCollege of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of Korea; Corresponding authors. Tel.: +82 31 4005805; fax: +82 31 4005958 (Ok-Nam Bae); Tel.: +82 31 4005808; fax: +82 31 4005958 (Jin-Ki Kim).College of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Republic of Korea; Corresponding authors. Tel.: +82 31 4005805; fax: +82 31 4005958 (Ok-Nam Bae); Tel.: +82 31 4005808; fax: +82 31 4005958 (Jin-Ki Kim).The short release half-life of carbon monoxide (CO) is a major obstacle to the effective therapeutic use of carbon monoxide-releasing molecule-2 (CORM-2). The potential of CORM-2-entrapped ultradeformable liposomes (CORM-2-UDLs) to enhance the release half-life of CO and alleviate skin inflammation was investigated in the present study. CORM-2-UDLs were prepared by using soy phosphatidylcholine to form lipid bilayers and Tween 80 as an edge activator. The deformability of CORM-2-UDLs was measured and compared with that of conventional liposomes by passing formulations through a filter device at a constant pressure. The release profile of CO from CORM-2-UDLs was evaluated by myoglobin assay. In vitro and in vivo anti-inflammatory effects of CORM-2-UDLs were assessed in lipopolysaccharide-stimulated macrophages and TPA-induced ear edema model, respectively. The deformability of the optimized CORM-2-UDLs was 2.3 times higher than conventional liposomes. CORM-2-UDLs significantly prolonged the release half-life of CO from 30 s in a CORM-2 solution to 21.6 min. CORM-2-UDLs demonstrated in vitro anti-inflammatory activity by decreasing nitrite production and pro-inflammatory cytokine levels. Furthermore, CORM-2-UDLs successfully ameliorated skin inflammation by reducing ear edema, pathological scores, neutrophil accumulation, and inflammatory cytokines expression. The results demonstrate that CORM-2-UDLs could be used as promising therapeutics against acute skin inflammation.http://www.sciencedirect.com/science/article/pii/S2211383520305931Carbon monoxideCORM-2Anti-inflammatory effectUltradeformable liposomesSkin inflammationEar edema |
| spellingShingle | Gwan-Yeong Lee Alam Zeb Eun-Hye Kim Beomseon Suh Young-Jun Shin Donghyun Kim Kyoung-Won Kim Yeong-Hwan Choe Ho-Ik Choi Cheol-Ho Lee Omer Salman Qureshi In-Bo Han Sun-Young Chang Ok-Nam Bae Jin-Ki Kim CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery Acta Pharmaceutica Sinica B Carbon monoxide CORM-2 Anti-inflammatory effect Ultradeformable liposomes Skin inflammation Ear edema |
| title | CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery |
| title_full | CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery |
| title_fullStr | CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery |
| title_full_unstemmed | CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery |
| title_short | CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO delivery |
| title_sort | corm 2 entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective co delivery |
| topic | Carbon monoxide CORM-2 Anti-inflammatory effect Ultradeformable liposomes Skin inflammation Ear edema |
| url | http://www.sciencedirect.com/science/article/pii/S2211383520305931 |
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