Cardiomyocyte dysfunction during the chronic phase of Chagas disease
Chagas disease, which is caused by the parasite Trypanosoma cruzi, is an important cause of heart failure. We investigated modifications in the cellular electrophysiological and calcium-handling characteristics of an infected mouse heart during the chronic phase of the disease. The patch-clamp techn...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2013-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200243&lng=en&tlng=en |
| _version_ | 1797715761906581504 |
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| author | Danilo Roman-Campos Policarpo Sales-Júnior Hugo Leonardo Duarte Eneas Ricardo Gomes Silvia Guatimosim Catherine Ropert Ricardo Tostes Gazzinelli Jader Santos Cruz |
| author_facet | Danilo Roman-Campos Policarpo Sales-Júnior Hugo Leonardo Duarte Eneas Ricardo Gomes Silvia Guatimosim Catherine Ropert Ricardo Tostes Gazzinelli Jader Santos Cruz |
| author_sort | Danilo Roman-Campos |
| collection | DOAJ |
| description | Chagas disease, which is caused by the parasite Trypanosoma cruzi, is an important cause of heart failure. We investigated modifications in the cellular electrophysiological and calcium-handling characteristics of an infected mouse heart during the chronic phase of the disease. The patch-clamp technique was used to record action potentials (APs) and L-type Ca2+ and transient outward K+ currents. [Ca2+]i changes were determined using confocal microscopy. Infected ventricular cells showed prolonged APs, reduced transient outward K+ and L-type Ca2+ currents and reduced Ca2+ release from the sarcoplasmic reticulum. Thus, the chronic phase of Chagas disease is characterised by cardiomyocyte dysfunction, which could lead to heart failure. |
| first_indexed | 2024-03-12T08:11:32Z |
| format | Article |
| id | doaj.art-17a00f86b0b34f8a8442419fde5e15aa |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:11:32Z |
| publishDate | 2013-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-17a00f86b0b34f8a8442419fde5e15aa2023-09-02T19:06:57ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602013-04-01108224324510.1590/0074-0276108022013019S0074-02762013000200243Cardiomyocyte dysfunction during the chronic phase of Chagas diseaseDanilo Roman-CamposPolicarpo Sales-JúniorHugo Leonardo DuarteEneas Ricardo GomesSilvia GuatimosimCatherine RopertRicardo Tostes GazzinelliJader Santos CruzChagas disease, which is caused by the parasite Trypanosoma cruzi, is an important cause of heart failure. We investigated modifications in the cellular electrophysiological and calcium-handling characteristics of an infected mouse heart during the chronic phase of the disease. The patch-clamp technique was used to record action potentials (APs) and L-type Ca2+ and transient outward K+ currents. [Ca2+]i changes were determined using confocal microscopy. Infected ventricular cells showed prolonged APs, reduced transient outward K+ and L-type Ca2+ currents and reduced Ca2+ release from the sarcoplasmic reticulum. Thus, the chronic phase of Chagas disease is characterised by cardiomyocyte dysfunction, which could lead to heart failure.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200243&lng=en&tlng=enChagas diseasecalcium currentaction potentialpotassium currentintracellular calciumTrypanosoma cruzi |
| spellingShingle | Danilo Roman-Campos Policarpo Sales-Júnior Hugo Leonardo Duarte Eneas Ricardo Gomes Silvia Guatimosim Catherine Ropert Ricardo Tostes Gazzinelli Jader Santos Cruz Cardiomyocyte dysfunction during the chronic phase of Chagas disease Memorias do Instituto Oswaldo Cruz Chagas disease calcium current action potential potassium current intracellular calcium Trypanosoma cruzi |
| title | Cardiomyocyte dysfunction during the chronic phase of Chagas disease |
| title_full | Cardiomyocyte dysfunction during the chronic phase of Chagas disease |
| title_fullStr | Cardiomyocyte dysfunction during the chronic phase of Chagas disease |
| title_full_unstemmed | Cardiomyocyte dysfunction during the chronic phase of Chagas disease |
| title_short | Cardiomyocyte dysfunction during the chronic phase of Chagas disease |
| title_sort | cardiomyocyte dysfunction during the chronic phase of chagas disease |
| topic | Chagas disease calcium current action potential potassium current intracellular calcium Trypanosoma cruzi |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200243&lng=en&tlng=en |
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