Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles
Existing optogenetic silencing methods affect membrane potential, biochemistry or protein integrity. Here, the authors demonstrate an approach for silencing synaptic transmission that combines fast activation and reversibility, by using nondisruptive, reversible, light-evoked clustering of synaptic...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-35324-z |
| _version_ | 1797977318906396672 |
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| author | Dennis Vettkötter Martin Schneider Brady D. Goulden Holger Dill Jana Liewald Sandra Zeiler Julia Guldan Yilmaz Arda Ateş Shigeki Watanabe Alexander Gottschalk |
| author_facet | Dennis Vettkötter Martin Schneider Brady D. Goulden Holger Dill Jana Liewald Sandra Zeiler Julia Guldan Yilmaz Arda Ateş Shigeki Watanabe Alexander Gottschalk |
| author_sort | Dennis Vettkötter |
| collection | DOAJ |
| description | Existing optogenetic silencing methods affect membrane potential, biochemistry or protein integrity. Here, the authors demonstrate an approach for silencing synaptic transmission that combines fast activation and reversibility, by using nondisruptive, reversible, light-evoked clustering of synaptic vesicles, which they validate in Caenorhabditis elegans, zebrafish, and murine cell culture. |
| first_indexed | 2024-04-11T05:06:03Z |
| format | Article |
| id | doaj.art-17a2ddfe2a2946ca86d655ad5e526bc7 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T05:06:03Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-17a2ddfe2a2946ca86d655ad5e526bc72022-12-25T12:21:11ZengNature PortfolioNature Communications2041-17232022-12-0113111710.1038/s41467-022-35324-zRapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesiclesDennis Vettkötter0Martin Schneider1Brady D. Goulden2Holger Dill3Jana Liewald4Sandra Zeiler5Julia Guldan6Yilmaz Arda Ateş7Shigeki Watanabe8Alexander Gottschalk9Buchmann Institute for Molecular Life Sciences, Goethe UniversityBuchmann Institute for Molecular Life Sciences, Goethe UniversityDepartment of Cell Biology and Solomon H. Snyder Department of Neuroscience, Johns Hopkins UniversityBuchmann Institute for Molecular Life Sciences, Goethe UniversityBuchmann Institute for Molecular Life Sciences, Goethe UniversityBuchmann Institute for Molecular Life Sciences, Goethe UniversityBuchmann Institute for Molecular Life Sciences, Goethe UniversityBuchmann Institute for Molecular Life Sciences, Goethe UniversityDepartment of Cell Biology and Solomon H. Snyder Department of Neuroscience, Johns Hopkins UniversityBuchmann Institute for Molecular Life Sciences, Goethe UniversityExisting optogenetic silencing methods affect membrane potential, biochemistry or protein integrity. Here, the authors demonstrate an approach for silencing synaptic transmission that combines fast activation and reversibility, by using nondisruptive, reversible, light-evoked clustering of synaptic vesicles, which they validate in Caenorhabditis elegans, zebrafish, and murine cell culture.https://doi.org/10.1038/s41467-022-35324-z |
| spellingShingle | Dennis Vettkötter Martin Schneider Brady D. Goulden Holger Dill Jana Liewald Sandra Zeiler Julia Guldan Yilmaz Arda Ateş Shigeki Watanabe Alexander Gottschalk Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles Nature Communications |
| title | Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles |
| title_full | Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles |
| title_fullStr | Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles |
| title_full_unstemmed | Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles |
| title_short | Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles |
| title_sort | rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles |
| url | https://doi.org/10.1038/s41467-022-35324-z |
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