Low expression of CD39 and CD73 genes in centenarians compared with octogenarians
Abstract Ageing involves a progressive decline of the body’s regulatory systems including immune system. Adenosine regulates immune function by interaction with its receptors, mainly adenosine A2A receptor, present on the surface of immune cells. Furthermore, cellular response to this nucleoside is...
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | Immunity & Ageing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12979-017-0094-3 |
| _version_ | 1819002092504743936 |
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| author | Almudena Crooke Juan Martínez-Henández Joaquín Martínez-López Alfonso Cruz-Jentoft Fernando Huete-Toral Jesús Pintor |
| author_facet | Almudena Crooke Juan Martínez-Henández Joaquín Martínez-López Alfonso Cruz-Jentoft Fernando Huete-Toral Jesús Pintor |
| author_sort | Almudena Crooke |
| collection | DOAJ |
| description | Abstract Ageing involves a progressive decline of the body’s regulatory systems including immune system. Adenosine regulates immune function by interaction with its receptors, mainly adenosine A2A receptor, present on the surface of immune cells. Furthermore, cellular response to this nucleoside is highly dependent on its extracellular concentration that is regulated by ecto-enzymes such as CD39 and CD73. Therefore, the aim of this study was to investigate the effect of age on adenosine A2A receptor, CD39 and CD73 gene expression. Changes in mRNA were measured by quantitative PCR from peripheral blood of young, middle-aged and older adults as well as centenarians. Centenarians showed a prominent decrease of CD39 and CD73 mRNA in comparison with older adults. Regarding to adenosine A2A receptor, we detected two subgroups of centenarians with high and low level of transcript. Additionally, adenosine A2A receptor mRNA level of centenarians, did not correlate with their cognitive impairment. In summary, our pilot study suggests that unlike of adenosine A2A receptor, the level of CD39 as well as CD73 mRNA could be a hallmark of successful human ageing. |
| first_indexed | 2024-12-20T22:59:37Z |
| format | Article |
| id | doaj.art-17b201d1d4154f55b26da18440bf49a6 |
| institution | Directory Open Access Journal |
| issn | 1742-4933 |
| language | English |
| last_indexed | 2024-12-20T22:59:37Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Immunity & Ageing |
| spelling | doaj.art-17b201d1d4154f55b26da18440bf49a62022-12-21T19:24:01ZengBMCImmunity & Ageing1742-49332017-05-011411510.1186/s12979-017-0094-3Low expression of CD39 and CD73 genes in centenarians compared with octogenariansAlmudena Crooke0Juan Martínez-Henández1Joaquín Martínez-López2Alfonso Cruz-Jentoft3Fernando Huete-Toral4Jesús Pintor5Department of Biochemistry and Molecular Biology IV, Faculty of Optics and Optometry, Universidad Complutense de MadridGeneral Medical Council of Spain, Fundación para la FormaciónDepartment of Hematology, Hospital Universitario 12 de OctubreDepartment of Geriatrics, Hospital Universitario Ramón y CajalDepartment of Biochemistry and Molecular Biology IV, Faculty of Optics and Optometry, Universidad Complutense de MadridDepartment of Biochemistry and Molecular Biology IV, Faculty of Optics and Optometry, Universidad Complutense de MadridAbstract Ageing involves a progressive decline of the body’s regulatory systems including immune system. Adenosine regulates immune function by interaction with its receptors, mainly adenosine A2A receptor, present on the surface of immune cells. Furthermore, cellular response to this nucleoside is highly dependent on its extracellular concentration that is regulated by ecto-enzymes such as CD39 and CD73. Therefore, the aim of this study was to investigate the effect of age on adenosine A2A receptor, CD39 and CD73 gene expression. Changes in mRNA were measured by quantitative PCR from peripheral blood of young, middle-aged and older adults as well as centenarians. Centenarians showed a prominent decrease of CD39 and CD73 mRNA in comparison with older adults. Regarding to adenosine A2A receptor, we detected two subgroups of centenarians with high and low level of transcript. Additionally, adenosine A2A receptor mRNA level of centenarians, did not correlate with their cognitive impairment. In summary, our pilot study suggests that unlike of adenosine A2A receptor, the level of CD39 as well as CD73 mRNA could be a hallmark of successful human ageing.http://link.springer.com/article/10.1186/s12979-017-0094-3AgeingCentenarianAdenosine receptorEcto-nucleotidase |
| spellingShingle | Almudena Crooke Juan Martínez-Henández Joaquín Martínez-López Alfonso Cruz-Jentoft Fernando Huete-Toral Jesús Pintor Low expression of CD39 and CD73 genes in centenarians compared with octogenarians Immunity & Ageing Ageing Centenarian Adenosine receptor Ecto-nucleotidase |
| title | Low expression of CD39 and CD73 genes in centenarians compared with octogenarians |
| title_full | Low expression of CD39 and CD73 genes in centenarians compared with octogenarians |
| title_fullStr | Low expression of CD39 and CD73 genes in centenarians compared with octogenarians |
| title_full_unstemmed | Low expression of CD39 and CD73 genes in centenarians compared with octogenarians |
| title_short | Low expression of CD39 and CD73 genes in centenarians compared with octogenarians |
| title_sort | low expression of cd39 and cd73 genes in centenarians compared with octogenarians |
| topic | Ageing Centenarian Adenosine receptor Ecto-nucleotidase |
| url | http://link.springer.com/article/10.1186/s12979-017-0094-3 |
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