Quality Control of Bacterial Extracellular Vesicles with Total Protein Content Assay, Nanoparticles Tracking Analysis, and Capillary Electrophoresis

Quality Control of Bacterial Extracellular Vesicles with Total Protein Content Assay, Nanoparticles Tracking Analysis, and Capillary Electrophoresis

Extracellular vesicles (EVs) were isolated from <i>Pectobacterium zantedeschiae</i> culturing media using direct ultracentrifugation (UC), iodixanol cushion ultracentrifugation (ICUC), and iodixanol density gradient ultracentrifugation (IDGUC) techniques. The isolates were characterized...

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Bibliographic Details
Main Authors: Aleksandra Steć, Joanna Jońca, Krzysztof Waleron, Małgorzata Waleron, Agata Płoska, Leszek Kalinowski, Bartosz Wielgomas, Szymon Dziomba
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:International Journal of Molecular Sciences
Subjects:
electrophoresis
extracellular vesicles
<i>Pectobacterium</i>
purity
subpopulations
ultracentrifugation
Online Access:https://www.mdpi.com/1422-0067/23/8/4347

Internet

https://www.mdpi.com/1422-0067/23/8/4347

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