Comparing chemical transfection, electroporation, and lentiviral vector transduction to achieve optimal transfection conditions in the Vero cell line

Comparing chemical transfection, electroporation, and lentiviral vector transduction to achieve optimal transfection conditions in the Vero cell line

Abstract Background Transfection is an important analytical method for studying gene expression in the cellular environment. There are some barriers to efficient DNA transfection in host cells, including circumventing the plasma membrane, escaping endosomal compartmentalization, autophagy, immune se...

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Bibliographic Details
Main Authors: Parisa Jamour, Abbas Jamali, Arash Ghalyanchi Langeroudi, Behrouz Ebadi Sharafabad, Asghar Abdoli
Format: Article
Language:English
Published: BMC 2024-05-01
Series:BMC Molecular and Cell Biology
Subjects:
Vero cell line
Chemical transfection reagents
Transfection efficiency
Electroporation
TurboFect
Lentivirus
Online Access:https://doi.org/10.1186/s12860-024-00511-x

Internet

https://doi.org/10.1186/s12860-024-00511-x

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