Minimal primer and primer-free SELEX protocols for selection of aptamers from random DNA libraries
Standard systematic evolution of ligands by exponential enrichment (SELEX) protocols require libraries that contain two primers, one on each side of a central random domain, which allow amplification of target-bound sequences via PCR or RT-PCR. However, these primer sequences cause nonspecific bindi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2008-03-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112689 |